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N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2022, 卷号: 160, 页码: 8
作者:  Tian, Miao;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Liang, Cuiyi;  Li, Zhibing;  Yang, LingMei;  Liu, Tao;  Li, Ming;  Luo, Wen
收藏  |  浏览/下载：111/0  |  提交时间：2023/11/21
N-glycosylation  Loop structure  Rhizomucor miehei lipase  Methanol tolerance  Biodiesel  
Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design 期刊论文
BIORESOURCE TECHNOLOGY, 2022, 卷号: 348, 页码: 9
作者:  Tian, Miao;  Yang, Lingmei;  Lv, Pengmei;  Wang, Zhiyuan;  Fu, Junying;  Miao, Changlin;  Li, Zhibing;  Li, Lianhua;  Liu, Tao;  Du, Wenyi;  Luo, Wen
收藏  |  浏览/下载：97/0  |  提交时间：2023/11/21
Biodiesel  Methanol tolerance  N-glycosylation  Molecular dynamics  Rhizomucor miehei lipase  
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
作者:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
收藏  |  浏览/下载：399/0  |  提交时间：2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:  Tian, Miao;  Fu, Junying;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  He, Dong;  Li, Zhibing;  Liu, Tao;  Li, Ming;  Luo, Wen
收藏  |  浏览/下载：240/0  |  提交时间：2021/10/27
Rhizomucor miehei lipase  N-linked glycosylation site  Thermostability  Methanol tolerance  Biodiesel  
Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:  Zhang, Jun;  Chen, Xiaoyan;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
收藏  |  浏览/下载：266/0  |  提交时间：2021/10/27
Lipase  Bionic-immobilization  Biodiesel  Bio-silicification  Molecular dynamics simulation  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
作者:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
收藏  |  浏览/下载：317/0  |  提交时间：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil 期刊论文
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2021, 页码: 9
作者:  Zhang, Jun;  Chen, Huanjun;  Wang, Zhiyuan;  Xu, Huijuan;  Luo, Wen;  Xu, Jingliang;  Lv, Pengmei
收藏  |  浏览/下载：281/0  |  提交时间：2021/10/27
biodiesel  Escherichia coli  Lipase  
Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2020, 页码: 13
作者:  Zhang, Jun;  Tian, Miao;  Chen, Xiaoyan;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
收藏  |  浏览/下载：280/0  |  提交时间：2021/11/01
Lipase  Lipase chaperone  Co-expression  Escherichia coli  Biodiesel  
High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil 期刊论文
3 BIOTECH, 2020, 卷号: 10, 期号: 12, 页码: 9
作者:  Zhang, Jun;  Tian, Miao;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
收藏  |  浏览/下载：266/0  |  提交时间：2021/11/01
Thermophilic lipase  Escherichia coli  Enzymatic hydrolysis  Rapeseed oil  
Effect of Propeptide Variation on Properties of Rhizomucor miehei Lipase (vol 12, pg 330, 2018) 期刊论文
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY, 2018, 卷号: 12, 期号: 6, 页码: 572-572
作者:  Luo, Wen;  He, Dong;  Fu, Junying;  Lv, Pengmei;  Yuan, Zhenhong;  Alam, Md. Asraful;  Wang, Zhiyuan;  Huang, Shaowei
收藏  |  浏览/下载：153/0  |  提交时间：2020/10/29