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N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2022, 卷号: 160, 页码: 8
作者:  Tian, Miao;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Liang, Cuiyi;  Li, Zhibing;  Yang, LingMei;  Liu, Tao;  Li, Ming;  Luo, Wen
收藏  |  浏览/下载：109/0  |  提交时间：2023/11/21
N-glycosylation  Loop structure  Rhizomucor miehei lipase  Methanol tolerance  Biodiesel  
Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design 期刊论文
BIORESOURCE TECHNOLOGY, 2022, 卷号: 348, 页码: 9
作者:  Tian, Miao;  Yang, Lingmei;  Lv, Pengmei;  Wang, Zhiyuan;  Fu, Junying;  Miao, Changlin;  Li, Zhibing;  Li, Lianhua;  Liu, Tao;  Du, Wenyi;  Luo, Wen
收藏  |  浏览/下载：96/0  |  提交时间：2023/11/21
Biodiesel  Methanol tolerance  N-glycosylation  Molecular dynamics  Rhizomucor miehei lipase  
Improved methanol tolerance of Rhizomucor miehei lipase based on N?glycosylation within the α-helix region and its application in biodiesel production 期刊论文
Biotechnology for Biofuels, 2021, 卷号: 14, 期号: 1
作者:  Tian,Miao;  Yang,Lingmei;  Wang,Zhiyuan;  Lv,Pengmei;  Fu,Junying;  Miao,Changlin;  Li,Ming;  Liu,Tao;  Luo,Wen
收藏  |  浏览/下载：143/0  |  提交时间：2022/09/20
Rhizomucor miehei lipase  α-Helix region  N?Glycosylation  Methanol tolerance  Biodiesel  
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
作者:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
收藏  |  浏览/下载：396/0  |  提交时间：2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:  Tian, Miao;  Fu, Junying;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  He, Dong;  Li, Zhibing;  Liu, Tao;  Li, Ming;  Luo, Wen
收藏  |  浏览/下载：240/0  |  提交时间：2021/10/27
Rhizomucor miehei lipase  N-linked glycosylation site  Thermostability  Methanol tolerance  Biodiesel  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
作者:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
收藏  |  浏览/下载：316/0  |  提交时间：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris 期刊论文
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2020, 页码: 10
作者:  Tian, Miao;  Wang, Zhi-Yuan;  Fu, Jun-Ying;  Li, Hui-Wen;  Zhang, Jun;  Zhang, Xu-Feng;  Luo, Wen;  Lv, Peng-Mei
收藏  |  浏览/下载：232/0  |  提交时间：2021/10/27
Box-Behnken design  crude glycerol  impurities  lipase production  Pichia pastoris  
Effect of Propeptide Variation on Properties of Rhizomucor miehei Lipase (vol 12, pg 330, 2018) 期刊论文
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY, 2018, 卷号: 12, 期号: 6, 页码: 572-572
作者:  Luo, Wen;  He, Dong;  Fu, Junying;  Lv, Pengmei;  Yuan, Zhenhong;  Alam, Md. Asraful;  Wang, Zhiyuan;  Huang, Shaowei
收藏  |  浏览/下载：151/0  |  提交时间：2020/10/29
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
作者:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
收藏  |  浏览/下载：141/0  |  提交时间：2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
米黑根毛霉脂肪酶在毕赤酵母中的高效表达及酶学性质研究 期刊论文
林产化学与工业, 2016, 期号: 1, 页码: 135-140
作者:  罗文;  王治元;  苗长林;  吕鹏梅;  何东;  李惠文;  杨玲梅;  袁振宏
Adobe PDF(688Kb)  |  收藏  |  浏览/下载：445/103  |  提交时间：2017/12/31
米黑根毛霉脂肪酶  毕赤酵母  密码子优化  酶学性质