Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil

doi:10.1002/bab.2247

GIEC OpenIR

	Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil
	Zhang, Jun 1,2,3; Chen, Huanjun 1,3; Wang, Zhiyuan 1; Xu, Huijuan1 ; Luo, Wen1 ; Xu, Jingliang1,4 ; Lv, Pengmei1
	2021-09-14
发表期刊	BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
ISSN	0885-4513
页码	9
通讯作者	Luo, Wen(luowen@ms.giec.ac.cn) ; Lv, Pengmei(lvpm@ms.giec.ac.cn)
摘要	Due to its simple, less by-product and environment friendly properties, enzymatic transesterification of oil with short-chain alcohol to biodiesel, fatty acid methyl esters (FAMEs) is considered to be a promising way of green production and has attracted much attention. In this study, FAMEs were synthesized by an enzymatic method with recombinant lipase as catalysts. A thermophilic Bacillus thermocatenulatus lipase 2 (BTL2) was overexpressed in Escherichia coli BL21(DE3) through relative and quantitative analysis using real-time quantitative PCR. The results suggested that the BTL2 gene was overexpressed in E. coli at the mRNA level, and the recombinant strain harboring a high-copy number vectors was selected and applied to fermentation to produce BTL2 with enzyme activity of 35.54 U/mg cells. The recombinant BTL2 solution exhibited excellent resistance to neutral pH, high temperature, and organic solvents after a certain treatment. Finally, the effects of enzymatic transesterification for preparing biodiesel were studied, using rapeseed oil as raw material, as well as BTL2 solution as catalysts, which resulted in 86.04% yield of FAMEs under 50 degrees C for 36 h. The liquid BTL2 was directly used to prepare FAMEs at a higher temperature efficiently, making the thermophilic BTL2 had the potential application value in biodiesel reproduction subsequently.
关键词	biodiesel Escherichia coli Lipase
DOI	10.1002/bab.2247
关键词[WOS]	HIGH-LEVEL EXPRESSION ; THERMOALKALOPHILIC LIPASE
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFB1504003] ; Projects of International Cooperation and Exchanges NSFC[51861145103] ; Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Guangdong Special Support Program[2017TX04Z109] ; National Natural Science Foundation of China[51606201]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
项目资助者	National Key Research and Development Program of China ; Projects of International Cooperation and Exchanges NSFC ; Natural Science Foundation of Guangdong Province of China ; Guangdong Special Support Program ; National Natural Science Foundation of China
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000695671500001
出版者	WILEY
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/33894
专题	中国科学院广州能源研究所
通讯作者	Luo, Wen; Lv, Pengmei
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 2.Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Lab Marine Biol Prod,Guangdon, Coll Food Sci & Technol,Key Lab Adv Proc Aquat Pr, Zhanjiang, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Zhengzhou Univ, Sch Chem Engn, Zhengzhou, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Zhang, Jun,Chen, Huanjun,Wang, Zhiyuan,et al. Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2021:9.
APA	Zhang, Jun.,Chen, Huanjun.,Wang, Zhiyuan.,Xu, Huijuan.,Luo, Wen.,...&Lv, Pengmei.(2021).Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,9.
MLA	Zhang, Jun,et al."Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2021):9.