Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design | |
Tian, Miao1,2; Yang, Lingmei1 ; Lv, Pengmei1; Wang, Zhiyuan1; Fu, Junying1; Miao, Changlin1; Li, Zhibing1; Li, Lianhua1; Liu, Tao3; Du, Wenyi4; Luo, Wen1
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| 2022-03-01 | |
| Source Publication | BIORESOURCE TECHNOLOGY
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| ISSN | 0960-8524 |
| Volume | 348Pages:9 |
| Corresponding Author | Luo, Wen(luowen@ms.giec.ac.cn) |
| Abstract | Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production. A semirational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT). Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT. The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT. Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance. N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil). The engineered ProRML mutant has high potential for commercial biodiesel production. |
| Keyword | Biodiesel Methanol tolerance N-glycosylation Molecular dynamics Rhizomucor miehei lipase |
| DOI | 10.1016/j.biortech.2022.126769 |
| WOS Keyword | WASTE COOKING OIL ; N-GLYCOSYLATION ; EXPRESSION |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[51903236] ; National Natural Science Foundation of China[51806225] ; Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Natural Science Foundation of Guangdong Province of China[2021A1515010325] |
| WOS Research Area | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| Funding Organization | National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province of China |
| WOS Subject | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS ID | WOS:000800442200002 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/36681 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Luo, Wen |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Guangdong Pharmaceut Univ, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Peoples R China 4.Sichuan MoDe Technol Co Ltd, Chengdu 610000, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Tian, Miao,Yang, Lingmei,Lv, Pengmei,et al. Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design[J]. BIORESOURCE TECHNOLOGY,2022,348:9. |
| APA | Tian, Miao.,Yang, Lingmei.,Lv, Pengmei.,Wang, Zhiyuan.,Fu, Junying.,...&Luo, Wen.(2022).Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.BIORESOURCE TECHNOLOGY,348,9. |
| MLA | Tian, Miao,et al."Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design".BIORESOURCE TECHNOLOGY 348(2022):9. |
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