Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design

doi:10.1016/j.biortech.2022.126769

GIEC OpenIR

	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design
	Tian, Miao 1,2; Yang, Lingmei1 ; Lv, Pengmei1 ; Wang, Zhiyuan 1; Fu, Junying 1; Miao, Changlin 1; Li, Zhibing1 ; Li, Lianhua1 ; Liu, Tao 3; Du, Wenyi 4; Luo, Wen1
	2022-03-01
发表期刊	BIORESOURCE TECHNOLOGY
ISSN	0960-8524
卷号	348 页码:9
通讯作者	Luo, Wen(luowen@ms.giec.ac.cn)
摘要	Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production. A semirational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT). Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT. The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT. Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance. N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil). The engineered ProRML mutant has high potential for commercial biodiesel production.
关键词	Biodiesel Methanol tolerance N-glycosylation Molecular dynamics Rhizomucor miehei lipase
DOI	10.1016/j.biortech.2022.126769
关键词[WOS]	WASTE COOKING OIL ; N-GLYCOSYLATION ; EXPRESSION
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[51903236] ; National Natural Science Foundation of China[51806225] ; Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Natural Science Foundation of Guangdong Province of China[2021A1515010325]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
项目资助者	National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province of China
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000800442200002
出版者	ELSEVIER SCI LTD
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/36681
专题	中国科学院广州能源研究所
通讯作者	Luo, Wen
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Guangdong Pharmaceut Univ, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Peoples R China 4.Sichuan MoDe Technol Co Ltd, Chengdu 610000, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Tian, Miao,Yang, Lingmei,Lv, Pengmei,et al. Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design[J]. BIORESOURCE TECHNOLOGY,2022,348:9.
APA	Tian, Miao.,Yang, Lingmei.,Lv, Pengmei.,Wang, Zhiyuan.,Fu, Junying.,...&Luo, Wen.(2022).Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.BIORESOURCE TECHNOLOGY,348,9.
MLA	Tian, Miao,et al."Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design".BIORESOURCE TECHNOLOGY 348(2022):9.