High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil

doi:10.1007/s13205-020-02517-6

GIEC OpenIR

	High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil
	Zhang, Jun 1,3; Tian, Miao 1,3; Lv, Pengmei1 ; Luo, Wen1 ; Wang, Zhiyuan 1; Xu, Jingliang1,2 ; Wang, Zhongming1
	2020-11-10
发表期刊	3 BIOTECH
ISSN	2190-572X
卷号	10 期号:12 页码:9
通讯作者	Lv, Pengmei(lvpm@ms.giec.ac.cn) ; Xu, Jingliang(xujl@zzu.edu.cn)
摘要	Long-chain fatty acids are widely used in food and chemical industries, and the enzymatic preparation of fatty acids is considered an environmentally friendly process. In the present study, long-chain fatty acids were prepared by the enzymatic hydrolysis of rapeseed oil with a genetically engineered lipase. Because thermophilic lipase has strong stability at higher temperatures, it was more suitable for the industrial production of long-chain fatty acids. Therefore, the thermophilic lipase BTL2 from Geobacillus thermocatenulatus was efficiently expressed in E. coli BL21(DE3) cells with an enzyme activity of 39.50 U/mg followed by gene codon optimisation. Experimental results showed that the recombinant lipase BTL2 exhibited excellent resistance to certain organic solvents (n-hexane, benzene, ethanol, and butanol). The metal cation Ca2+ and the non-ionic surfactant Triton-100X enhanced enzyme activity by 7.36% and 56.21% respectively. Moreover, the acid value of the liberated long-chain fatty acids by hydrolysing rapeseed oil was approximately 161.64 mg KOH/g at 50 degrees C in 24 h, the hydrolytic conversion rate was 91.45%, and the productivity was approximately 6.735 mg KOH/g h. These results suggested that the recombinant lipase BTL2 has excellent hydrolytic performance for rapeseed oil and showed great potential for the enzymatic preparation of long-chain fatty acids.
关键词	Thermophilic lipase Escherichia coli Enzymatic hydrolysis Rapeseed oil
DOI	10.1007/s13205-020-02517-6
关键词[WOS]	METAL-ORGANIC FRAMEWORKS ; BACILLUS ; IMMOBILIZATION ; PURIFICATION ; AMYLASE ; PROTEIN ; BTL2
收录类别	SCI
语种	英语
资助项目	Guangdong Special Support Program[2017TX04Z109] ; National Natural Science Foundation of China[51606201] ; National Natural Science Foundation of China[51903236] ; Science and Technology Planning Project of Guangdong Province[2016A010104008] ; Natural Science Foundation of Guangdong Province[2017A010104010] ; National Key research and development program of China[2019YFB1504003] ; Projects of International Cooperation and Exchanges NSFC[51861145103]
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	Guangdong Special Support Program ; National Natural Science Foundation of China ; Science and Technology Planning Project of Guangdong Province ; Natural Science Foundation of Guangdong Province ; National Key research and development program of China ; Projects of International Cooperation and Exchanges NSFC
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000593779200005
出版者	SPRINGER HEIDELBERG
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/34829
专题	中国科学院广州能源研究所
通讯作者	Lv, Pengmei; Xu, Jingliang
作者单位	1.Chinese Acad Sci, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 2.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China 3.Univ China Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jun,Tian, Miao,Lv, Pengmei,et al. High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil[J]. 3 BIOTECH,2020,10(12):9.
APA	Zhang, Jun.,Tian, Miao.,Lv, Pengmei.,Luo, Wen.,Wang, Zhiyuan.,...&Wang, Zhongming.(2020).High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil.3 BIOTECH,10(12),9.
MLA	Zhang, Jun,et al."High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil".3 BIOTECH 10.12(2020):9.