N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production

doi:10.1016/j.enzmictec.2022.110072

GIEC OpenIR

	N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production
	Tian, Miao 1,2; Wang, Zhiyuan 1; Fu, Junying 1; Lv, Pengmei1 ; Liang, Cuiyi1 ; Li, Zhibing1 ; Yang, LingMei1 ; Liu, Tao 3; Li, Ming 1,4; Luo, Wen1
	2022-10-01
发表期刊	ENZYME AND MICROBIAL TECHNOLOGY
ISSN	0141-0229
卷号	160 页码:8
通讯作者	Luo, Wen(luowen@ms.giec.ac.cn)
摘要	The construction of methanol-resistant lipases with high catalytic activity is world-shattering for biodiesel production. A semi-rational method has been constructed to enhance the properties of Rhizomucor miehei lipase with propeptide (ProRML) by introducing N-glycosylation sites in the Loop structure. The enzyme activities of the mutants N288 (1448.89 +/- 68.64 U/mg) and N142 (1073.68 +/- 33.87 U/mg) increased to 56.09 and 41.56 times relative to that of wild type ProRML (WT, 25.83 +/- 0.73 U/mg), respectively. After incubation in 50 % methanol for 2.5 h, the residual activities of N314 and N174-1 were 95 % and 85%, which were higher than the WT (27 %). Additionally, the biodiesel yield of all mutants was increased after a one-time addition of methanol for 24 h. Among them, N288 increased the quantity of biodiesel from colza oil from 9.49 % to 88 %, and N314 increased the amount of biodiesel from waste soybean oil from 8.44% to 70%. This study provides an effective method to enhance the properties of lipase and improve its application potential in biodiesel production.
关键词	N-glycosylation Loop structure Rhizomucor miehei lipase Methanol tolerance Biodiesel
DOI	10.1016/j.enzmictec.2022.110072
关键词[WOS]	WASTE COOKING OIL ; LIQUID LIPASE ; STABILITY ; LOOPS ; EXPRESSION ; PROTEINS ; STATE ; ACID ; SITE
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Natural Science Foundation of Guangdong Province of China[2021A1515010325] ; National Natural Science Foundation of China[51903236] ; National Natural Science Foundation of China[51806225]
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	Natural Science Foundation of Guangdong Province of China ; National Natural Science Foundation of China
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000811258400003
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/36803
专题	中国科学院广州能源研究所
通讯作者	Luo, Wen
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Guangdong Pharmaceut Univ, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Peoples R China 4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Tian, Miao,Wang, Zhiyuan,Fu, Junying,et al. N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production[J]. ENZYME AND MICROBIAL TECHNOLOGY,2022,160:8.
APA	Tian, Miao.,Wang, Zhiyuan.,Fu, Junying.,Lv, Pengmei.,Liang, Cuiyi.,...&Luo, Wen.(2022).N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production.ENZYME AND MICROBIAL TECHNOLOGY,160,8.
MLA	Tian, Miao,et al."N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production".ENZYME AND MICROBIAL TECHNOLOGY 160(2022):8.