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Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
收藏  |  浏览/下载：257/0  |  提交时间：2017/10/13
Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Cation exchange resin catalysed biodiesel production from used cooking oil (UCO): Investigation of impurities effect 期刊论文
FUEL, 2016, 卷号: 181, 页码: 1058-1065
作者:  Fu, Junying;  Li, Zhibing;  Xing, Shiyou;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  Yuan, Zhenhong
收藏  |  浏览/下载：259/0  |  提交时间：2017/10/10
Biodiesel  Used Cooking Oil  Impurities  Cation Exchange Resin  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
作者:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
收藏  |  浏览/下载：145/0  |  提交时间：2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
收藏  |  浏览/下载：139/0  |  提交时间：2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization