Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris

doi:10.1080/10826068.2020.1870135

GIEC OpenIR

	Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris
	Tian, Miao 1,2; Wang, Zhi-Yuan 1; Fu, Jun-Ying 1; Li, Hui-Wen 1; Zhang, Jun 1,2; Zhang, Xu-Feng 2,3; Luo, Wen1 ; Lv, Peng-Mei1
	2020-12-30
发表期刊	PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
ISSN	1082-6068
页码	10
通讯作者	Luo, Wen(luowen@ms.giec.ac.cn) ; Lv, Peng-Mei(lvpm@ms.giec.ac.cn)
摘要	Crude glycerol, a by-product of biodiesel production, was employed as the carbon source to produce lipase using Pichia pastoris. Under identical fermentation conditions, cell growth and lipase activity were improved using crude glycerol instead of pure glycerol. The impacts of crude glycerol impurities (methyl ester, grease, glycerol, methanol, and metal ions Na+, Ca2+, and Fe3+) on lipase production were investigated. Impurities accelerated P. pastoris entering the stationary phase. Na+, Ca2+, and grease in waste crude glycerol were the main factors influencing higher lipase activity. Through response surface optimization of Ca2+, Na+, and grease concentrations, lipase activity reached 1437 U/mL (15,977 U/mg), which was 2.5 times that of the control. This study highlights the economical and highly efficient valorization of crude glycerol, demonstrating its possible utilization as a carbon source to produce lipase by P. pastoris without pretreatment.
关键词	Box-Behnken design crude glycerol impurities lipase production Pichia pastoris
DOI	10.1080/10826068.2020.1870135
收录类别	SCI
语种	英语
资助项目	China National Key R&D Program during the 13th Five-year Plan Period[2017YFD0601003] ; State Key Program of National Nature Science Foundation of China[51903236] ; State Key Program of National Nature Science Foundation of China[51806225] ; State Key Program of National Nature Science Foundation of China[51606201] ; Guangzhou Science and technology planning project[201804010125] ; Science and technology planning project of Guangdong province[2016A010104008]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
项目资助者	China National Key R&D Program during the 13th Five-year Plan Period ; State Key Program of National Nature Science Foundation of China ; Guangzhou Science and technology planning project ; Science and technology planning project of Guangdong province
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000607424900001
出版者	TAYLOR & FRANCIS INC
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/32508
专题	中国科学院广州能源研究所
通讯作者	Luo, Wen; Lv, Peng-Mei
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Sci & Technol China, Nano Sci & Technol Inst, Suzhou, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Tian, Miao,Wang, Zhi-Yuan,Fu, Jun-Ying,et al. Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2020:10.
APA	Tian, Miao.,Wang, Zhi-Yuan.,Fu, Jun-Ying.,Li, Hui-Wen.,Zhang, Jun.,...&Lv, Peng-Mei.(2020).Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,10.
MLA	Tian, Miao,et al."Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2020):10.