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N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2022, 卷号: 160, 页码: 8
作者:  Tian, Miao;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Liang, Cuiyi;  Li, Zhibing;  Yang, LingMei;  Liu, Tao;  Li, Ming;  Luo, Wen
收藏  |  浏览/下载：133/0  |  提交时间：2023/11/21
N-glycosylation  Loop structure  Rhizomucor miehei lipase  Methanol tolerance  Biodiesel  
Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design 期刊论文
BIORESOURCE TECHNOLOGY, 2022, 卷号: 348, 页码: 9
作者:  Tian, Miao;  Yang, Lingmei;  Lv, Pengmei;  Wang, Zhiyuan;  Fu, Junying;  Miao, Changlin;  Li, Zhibing;  Li, Lianhua;  Liu, Tao;  Du, Wenyi;  Luo, Wen
收藏  |  浏览/下载：114/0  |  提交时间：2023/11/21
Biodiesel  Methanol tolerance  N-glycosylation  Molecular dynamics  Rhizomucor miehei lipase  
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
作者:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
收藏  |  浏览/下载：431/0  |  提交时间：2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:  Tian, Miao;  Fu, Junying;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  He, Dong;  Li, Zhibing;  Liu, Tao;  Li, Ming;  Luo, Wen
收藏  |  浏览/下载：248/0  |  提交时间：2021/10/27
Rhizomucor miehei lipase  N-linked glycosylation site  Thermostability  Methanol tolerance  Biodiesel  
Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:  Zhang, Jun;  Chen, Xiaoyan;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
收藏  |  浏览/下载：280/0  |  提交时间：2021/10/27
Lipase  Bionic-immobilization  Biodiesel  Bio-silicification  Molecular dynamics simulation  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
作者:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
收藏  |  浏览/下载：324/0  |  提交时间：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil 期刊论文
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2021, 页码: 9
作者:  Zhang, Jun;  Chen, Huanjun;  Wang, Zhiyuan;  Xu, Huijuan;  Luo, Wen;  Xu, Jingliang;  Lv, Pengmei
收藏  |  浏览/下载：297/0  |  提交时间：2021/10/27
biodiesel  Escherichia coli  Lipase  
Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2020, 页码: 13
作者:  Zhang, Jun;  Tian, Miao;  Chen, Xiaoyan;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
收藏  |  浏览/下载：296/0  |  提交时间：2021/11/01
Lipase  Lipase chaperone  Co-expression  Escherichia coli  Biodiesel  
Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase 期刊论文
ACS APPLIED MATERIALS & INTERFACES, 2018, 卷号: 10, 期号: 37, 页码: 30973-30982
作者:  Fu, Junying;  Wang, Zhiyuan;  Luo, Wen;  Xing, Shiyou;  Lv, Pengmei;  Wang, Zhongming;  Yuan, Zhenhong
收藏  |  浏览/下载：274/0  |  提交时间：2020/10/29
Sanger's reagent  immobilization  lipase  hydrolysis  enantioselectivity  
Natural albite as a novel solid basic catalyst for the effective synthesis of biodiesel: Characteristics and performance 期刊论文
ENERGY, 2017, 卷号: 141, 页码: 1650-1660
作者:  Wang, Jiayan;  Wang, Zhiyuan;  Yang, Lingmei;  Yang, Gaixiu;  Miao, Changlin;  Lv, Pengmei
收藏  |  浏览/下载：174/0  |  提交时间：2020/10/29
Natural albite  Solid base catalyst  Biodiesel  Transesterification