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N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2022, 卷号: 160, 页码: 8
Authors:  Tian, Miao;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Liang, Cuiyi;  Li, Zhibing;  Yang, LingMei;  Liu, Tao;  Li, Ming;  Luo, Wen
Favorite  |  View/Download：190/0  |  Submit date：2023/11/21
N-glycosylation  Loop structure  Rhizomucor miehei lipase  Methanol tolerance  Biodiesel  
Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design 期刊论文
BIORESOURCE TECHNOLOGY, 2022, 卷号: 348, 页码: 9
Authors:  Tian, Miao;  Yang, Lingmei;  Lv, Pengmei;  Wang, Zhiyuan;  Fu, Junying;  Miao, Changlin;  Li, Zhibing;  Li, Lianhua;  Liu, Tao;  Du, Wenyi;  Luo, Wen
Favorite  |  View/Download：178/0  |  Submit date：2023/11/21
Biodiesel  Methanol tolerance  N-glycosylation  Molecular dynamics  Rhizomucor miehei lipase  
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
Authors:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
Favorite  |  View/Download：490/0  |  Submit date：2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
Authors:  Tian, Miao;  Fu, Junying;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  He, Dong;  Li, Zhibing;  Liu, Tao;  Li, Ming;  Luo, Wen
Favorite  |  View/Download：315/0  |  Submit date：2021/10/27
Rhizomucor miehei lipase  N-linked glycosylation site  Thermostability  Methanol tolerance  Biodiesel  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
Authors:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
Favorite  |  View/Download：380/0  |  Submit date：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2020, 页码: 13
Authors:  Zhang, Jun;  Tian, Miao;  Chen, Xiaoyan;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
Favorite  |  View/Download：346/0  |  Submit date：2021/11/01
Lipase  Lipase chaperone  Co-expression  Escherichia coli  Biodiesel  
High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil 期刊论文
3 BIOTECH, 2020, 卷号: 10, 期号: 12, 页码: 9
Authors:  Zhang, Jun;  Tian, Miao;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
Favorite  |  View/Download：340/0  |  Submit date：2021/11/01
Thermophilic lipase  Escherichia coli  Enzymatic hydrolysis  Rapeseed oil