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Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
Authors:  Tian, Miao;  Fu, Junying;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  He, Dong;  Li, Zhibing;  Liu, Tao;  Li, Ming;  Luo, Wen
Favorite  |  View/Download：315/0  |  Submit date：2021/10/27
Rhizomucor miehei lipase  N-linked glycosylation site  Thermostability  Methanol tolerance  Biodiesel  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
Authors:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
Favorite  |  View/Download：380/0  |  Submit date：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris 期刊论文
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2020, 页码: 10
Authors:  Tian, Miao;  Wang, Zhi-Yuan;  Fu, Jun-Ying;  Li, Hui-Wen;  Zhang, Jun;  Zhang, Xu-Feng;  Luo, Wen;  Lv, Peng-Mei
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Box-Behnken design  crude glycerol  impurities  lipase production  Pichia pastoris  
Gene cloning and characterization of an organic solvent-stimulated beta-glucosidase and its application for the co-production of ethanol and succinic acid 期刊论文
CELLULOSE, 2019, 卷号: 26, 期号: 15, 页码: 8237-8248
Authors:  Liang, Cui Y.;  Xu, Jing L.;  Xu, Hui J.;  Qi, Wei;  Zhang, Yu;  Luo, Wen;  Chen, Xiao Y.;  Wang, Zhong M.;  Yuan, Zhen H.
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beta-Glucosidase  Organic solvent stimulation  Succinic acid  Ethanol  Co-fermentation  
Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2018, 卷号: 184, 期号: 1, 页码: 228-238
Authors:  Li, Hailong;  Wu, Hongli;  Jiang, Fengjiao;  Wu, Jinlian;  Xue, Yong;  Gan, Lihui;  Liu, Jian;  Long, Minnan
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Heterologous expression  Pichia pastoris  Xylan hydrolysis  Xylanases  Hypocrea orientalis  Xylo-oligosaccharides  
Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
Authors:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
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Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass 期刊论文
BIORESOURCES, 2017, 卷号: 12, 期号: 1, 页码: 434-447
Authors:  Wu, Hongli;  Xue, Yong;  Li, Hailong;  Gan, Lihui;  Liu, Jian;  Long, Minnan
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Aspergillus Niger  Acetyl Xylan Esterase  Pichia Pastoris  Characterization  Synergistic Cooperation  
Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass 期刊论文
BIORESOURCES, 2017, 卷号: 12, 期号: 1, 页码: 434-447
Authors:  Wu, Hongli;  Xue, Yong;  Li, Hailong;  Gan, Lihui;  Liu, Jian;  Long, Minnan
Favorite  |  View/Download：191/0  |  Submit date：2017/10/13
Aspergillus Niger  Acetyl Xylan Esterase  Pichia Pastoris  Characterization  Synergistic Cooperation  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
Authors:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
Favorite  |  View/Download：182/0  |  Submit date：2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
米黑根毛霉脂肪酶在毕赤酵母中的高效表达及酶学性质研究 期刊论文
林产化学与工业, 2016, 期号: 1, 页码: 135-140
Authors:  罗文;  王治元;  苗长林;  吕鹏梅;  何东;  李惠文;  杨玲梅;  袁振宏
View  |  Adobe PDF(688Kb)  |  Favorite  |  View/Download：596/149  |  Submit date：2017/12/31
米黑根毛霉脂肪酶  毕赤酵母  密码子优化  酶学性质