Gene cloning and characterization of an organic solvent-stimulated beta-glucosidase and its application for the co-production of ethanol and succinic acid

doi:10.1007/s10570-019-02477-y

GIEC OpenIR

	Gene cloning and characterization of an organic solvent-stimulated beta-glucosidase and its application for the co-production of ethanol and succinic acid
	Liang, Cui Y.1,2,3,4; Xu, Jing L.1,2,3; Xu, Hui J.1,2,3; Qi, Wei1,2,3 ; Zhang, Yu1,2,3 ; Luo, Wen1,2,3 ; Chen, Xiao Y.1,2,3,4; Wang, Zhong M.1,2,3; Yuan, Zhen H.1,2,3
	2019-10-01
发表期刊	CELLULOSE
ISSN	0969-0239
卷号	26 期号:15 页码:8237-8248
通讯作者	Qi, Wei(qiwei@ms.giec.ac.cn) ; Wang, Zhong M.(wangzm@ms.giec.ac.cn)
摘要	Enzymatic activity is a limiting factor for conducting the hydrolysis of lignocellulosic waste materials to generate renewable biofuels and biochemicals. A novel beta-glucosidase (BGL) gene from Hypocrea sp. W-63 was cloned and expressed in Pichia pastoris. The specific activity of the purified recombinant epB-BGL was 194.25 IU/mg using p-nitrophenyl-beta-D-glucoside (pNPG) as a substrate. The optimum pH and temperature for epB-BGL were 5.0 and 70 degrees C, respectively. The activity of recombinant epB-BGL could be stimulated by 210% with the addition of 10% (v/v) ethanol. Furthermore, other organic solvents were also able to stimulate the activity of the enzyme at concentrations of 1% or 10% (v/v). Due to this distinctive performance, epB-BGL was used to release fermentable sugars from a sugarcane bagasse hydrolysate for ethanol and succinic acid co-production. In the experimental range, succinic acid was reached to 13.3 g/L with a higher yield of 0.91 g/g glucose and the ethanol was at a concentration of approximately 1.41 g/L, respectively. In addition, the by-product acetate, which could potentially be converted to other chemicals, was also produced at 9.745 g/L. This work demonstrates that recombinant epB-BGL can take advantage of incomplete hydrolysis to convert fermentable sugars, suggesting its potential application in lignocellulose bioconversion.
关键词	beta-Glucosidase Organic solvent stimulation Succinic acid Ethanol Co-fermentation
DOI	10.1007/s10570-019-02477-y
关键词[WOS]	SUGARCANE BAGASSE HYDROLYSATE ; TRICHODERMA-REESEI ; ENZYMATIC-HYDROLYSIS ; EXPRESSION ; BIOMASS ; STRAIN ; BIOCONVERSION
收录类别	SCI
语种	英语
资助项目	Program of National Natural Science Foundation of China[51676193] ; Program of National Natural Science Foundation of China[51861145103] ; Youth Innovation Promotion Association, CAS[2017401] ; National Key Research and Development Program of China[2018YFC1901201] ; Bureau of International Cooperation, the Chinese Academy of Sciences[182344KYSB20160056] ; Guangdong science and technology research program[2017A010105018] ; Guangdong science and technology research program[2016A010104008] ; Municipal Science and Technology Program of Guangzhou[201804010187] ; Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and Modification[GXPSMM18ZD-01]
WOS研究方向	Materials Science ; Polymer Science
项目资助者	Program of National Natural Science Foundation of China ; Youth Innovation Promotion Association, CAS ; National Key Research and Development Program of China ; Bureau of International Cooperation, the Chinese Academy of Sciences ; Guangdong science and technology research program ; Municipal Science and Technology Program of Guangzhou ; Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and Modification
WOS类目	Materials Science, Paper & Wood ; Materials Science, Textiles ; Polymer Science
WOS记录号	WOS:000489757400003
出版者	SPRINGER
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/25935
专题	中国科学院广州能源研究所
通讯作者	Qi, Wei; Wang, Zhong M.
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Liang, Cui Y.,Xu, Jing L.,Xu, Hui J.,et al. Gene cloning and characterization of an organic solvent-stimulated beta-glucosidase and its application for the co-production of ethanol and succinic acid[J]. CELLULOSE,2019,26(15):8237-8248.
APA	Liang, Cui Y..,Xu, Jing L..,Xu, Hui J..,Qi, Wei.,Zhang, Yu.,...&Yuan, Zhen H..(2019).Gene cloning and characterization of an organic solvent-stimulated beta-glucosidase and its application for the co-production of ethanol and succinic acid.CELLULOSE,26(15),8237-8248.
MLA	Liang, Cui Y.,et al."Gene cloning and characterization of an organic solvent-stimulated beta-glucosidase and its application for the co-production of ethanol and succinic acid".CELLULOSE 26.15(2019):8237-8248.