Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis

doi:10.1007/s12010-017-2532-2

GIEC OpenIR

	Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis
	Li, Hailong 1,3,4; Wu, Hongli 2; Jiang, Fengjiao 2; Wu, Jinlian 2; Xue, Yong 2; Gan, Lihui 2; Liu, Jian 2; Long, Minnan 2
	2018
发表期刊	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN	0273-2289
卷号	184 期号:1 页码:228-238
通讯作者	Liu, Jian(jianliu@xmu.edu.cn) ; Long, Minnan(longmn@xmu.edu.cn)
摘要	A gene encoding glycoside hydrolase family 11 xylanase (HoXyn11B) from Hypocrea orientalis EU7-22 was expressed in Pichia pastoris with a high activity (413 IU/ml). HoXyn11B was partly N-glycosylated and appeared two protein bands (19-29 kDa) on SDS-PAGE. The recombinant enzyme exhibited optimal activity at pH 4.5 and 55 A degrees C, and retained more than 90% of the original activity after incubation at 50 A degrees C for 60 min. The determined apparent K (m) and V (max) values using beechwood xylan were 10.43 mg/ml and 3246.75 IU/mg, respectively. The modes of action of recombinant HoXyn11B on xylo-oligosaccharides (XOSs) and beechwood xylan were investigated by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), which indicated that the modes of action of HoXyn11B are different from HoXyn11A since it is able to release a significant amount of xylose from various substrates. This study provides an opportunity to better understand the hydrolysis mechanisms of xylan by xylanases from Trichoderma.
关键词	Heterologous expression Pichia pastoris Xylan hydrolysis Xylanases Hypocrea orientalis Xylo-oligosaccharides
DOI	10.1007/s12010-017-2532-2
关键词[WOS]	PICHIA-PASTORIS ; ENZYMATIC-HYDROLYSIS ; RECOMBINANT XYLANASE ; XYLOOLIGOSACCHARIDES ; OLIGOSACCHARIDES ; MONOSACCHARIDES ; HEMICELLULOSES ; PURIFICATION ; PROTEINS ; BINDING
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Guangdong Province, China[2016A030310124] ; National Natural Science Foundation of China[31600475] ; project of Guangzhou Science and Technology[201707010241] ; research fund from the Xiamen Southern Oceanographic Center[14GZP59HJ29] ; Fujian Provincial Department of Ocean and Fisheries[2015-27] ; President Fund of Xiamen University[20720150090]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
项目资助者	Natural Science Foundation of Guangdong Province, China ; National Natural Science Foundation of China ; project of Guangzhou Science and Technology ; research fund from the Xiamen Southern Oceanographic Center ; Fujian Provincial Department of Ocean and Fisheries ; President Fund of Xiamen University
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000419607700018
出版者	HUMANA PRESS INC
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/22884
专题	中国科学院广州能源研究所
通讯作者	Liu, Jian; Long, Minnan
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Xiamen Univ, Coll Energy, Xiamen 361102, Peoples R China 3.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Li, Hailong,Wu, Hongli,Jiang, Fengjiao,et al. Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2018,184(1):228-238.
APA	Li, Hailong.,Wu, Hongli.,Jiang, Fengjiao.,Wu, Jinlian.,Xue, Yong.,...&Long, Minnan.(2018).Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,184(1),228-238.
MLA	Li, Hailong,et al."Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 184.1(2018):228-238.