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中国科学院广州能源研究所机构知识库
Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
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31600475) [2]
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Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production
期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
Authors:
Tian, Miao
;
Fu, Junying
;
Wang, Zhiyuan
;
Miao, Changlin
;
Lv, Pengmei
;
He, Dong
;
Li, Zhibing
;
Liu, Tao
;
Li, Ming
;
Luo, Wen
Favorite
  |  
View/Download:330/0
  |  
Submit date:2021/10/27
Rhizomucor miehei lipase
N-linked glycosylation site
Thermostability
Methanol tolerance
Biodiesel
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide
期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
Authors:
Tian, Miao
;
Huang, Shaowei
;
Wang, Zhiyuan
;
Fu, Junying
;
Lv, Pengmei
;
Miao, Changlin
;
Liu, Tao
;
Yang, Lingmei
;
Luo, Wen
Favorite
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View/Download:393/0
  |  
Submit date:2021/11/01
Propeptide
RML
Enzyme activity
Directed saturation mutation
Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris
期刊论文
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2020, 页码: 10
Authors:
Tian, Miao
;
Wang, Zhi-Yuan
;
Fu, Jun-Ying
;
Li, Hui-Wen
;
Zhang, Jun
;
Zhang, Xu-Feng
;
Luo, Wen
;
Lv, Peng-Mei
Favorite
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View/Download:314/0
  |  
Submit date:2021/10/27
Box-Behnken design
crude glycerol
impurities
lipase production
Pichia pastoris
Gene cloning and characterization of an organic solvent-stimulated beta-glucosidase and its application for the co-production of ethanol and succinic acid
期刊论文
CELLULOSE, 2019, 卷号: 26, 期号: 15, 页码: 8237-8248
Authors:
Liang, Cui Y.
;
Xu, Jing L.
;
Xu, Hui J.
;
Qi, Wei
;
Zhang, Yu
;
Luo, Wen
;
Chen, Xiao Y.
;
Wang, Zhong M.
;
Yuan, Zhen H.
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  |  
View/Download:308/0
  |  
Submit date:2020/10/29
beta-Glucosidase
Organic solvent stimulation
Succinic acid
Ethanol
Co-fermentation
Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis
期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2018, 卷号: 184, 期号: 1, 页码: 228-238
Authors:
Li, Hailong
;
Wu, Hongli
;
Jiang, Fengjiao
;
Wu, Jinlian
;
Xue, Yong
;
Gan, Lihui
;
Liu, Jian
;
Long, Minnan
Favorite
  |  
View/Download:194/0
  |  
Submit date:2020/10/29
Heterologous expression
Pichia pastoris
Xylan hydrolysis
Xylanases
Hypocrea orientalis
Xylo-oligosaccharides
Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability
期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
Authors:
He, Dong
;
Luo, Wen
;
Wang, Zhiyuan
;
Lv, Pengmei
;
Yuan, Zhenhong
;
Huang, Shaowei
;
Xv, Jingliang
Favorite
  |  
View/Download:304/0
  |  
Submit date:2017/10/13
Directed Evolution
Membrane-blot
Methanol Tolerance
Thermostability
Rhizomucor Miehei Lipases
Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass
期刊论文
BIORESOURCES, 2017, 卷号: 12, 期号: 1, 页码: 434-447
Authors:
Wu, Hongli
;
Xue, Yong
;
Li, Hailong
;
Gan, Lihui
;
Liu, Jian
;
Long, Minnan
Favorite
  |  
View/Download:355/0
  |  
Submit date:2017/10/13
Aspergillus Niger
Acetyl Xylan Esterase
Pichia Pastoris
Characterization
Synergistic Cooperation
Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass
期刊论文
BIORESOURCES, 2017, 卷号: 12, 期号: 1, 页码: 434-447
Authors:
Wu, Hongli
;
Xue, Yong
;
Li, Hailong
;
Gan, Lihui
;
Liu, Jian
;
Long, Minnan
Favorite
  |  
View/Download:201/0
  |  
Submit date:2017/10/13
Aspergillus Niger
Acetyl Xylan Esterase
Pichia Pastoris
Characterization
Synergistic Cooperation
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region
期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
Authors:
Wang, Zhiyuan
;
Lv, Pengmei
;
Luo, Wen
;
Yuan, Zhenhong
;
He, Dong
Favorite
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View/Download:192/0
  |  
Submit date:2016/12/05
Enzyme Property
Intramolecular Chaperone
Propeptide Replacing
Protein Folding
Rhizomucor Miehei Lipases
米黑根毛霉脂肪酶在毕赤酵母中的高效表达及酶学性质研究
期刊论文
林产化学与工业, 2016, 期号: 1, 页码: 135-140
Authors:
罗文
;
王治元
;
苗长林
;
吕鹏梅
;
何东
;
李惠文
;
杨玲梅
;
袁振宏
Adobe PDF(688Kb)
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View/Download:616/157
  |  
Submit date:2017/12/31
米黑根毛霉脂肪酶
毕赤酵母
密码子优化
酶学性质