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Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:  Tian, Miao;  Fu, Junying;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  He, Dong;  Li, Zhibing;  Liu, Tao;  Li, Ming;  Luo, Wen
收藏  |  浏览/下载：242/0  |  提交时间：2021/10/27
Rhizomucor miehei lipase  N-linked glycosylation site  Thermostability  Methanol tolerance  Biodiesel  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
作者:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
收藏  |  浏览/下载：319/0  |  提交时间：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris 期刊论文
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2020, 页码: 10
作者:  Tian, Miao;  Wang, Zhi-Yuan;  Fu, Jun-Ying;  Li, Hui-Wen;  Zhang, Jun;  Zhang, Xu-Feng;  Luo, Wen;  Lv, Peng-Mei
收藏  |  浏览/下载：233/0  |  提交时间：2021/10/27
Box-Behnken design  crude glycerol  impurities  lipase production  Pichia pastoris  
Gene cloning and characterization of an organic solvent-stimulated beta-glucosidase and its application for the co-production of ethanol and succinic acid 期刊论文
CELLULOSE, 2019, 卷号: 26, 期号: 15, 页码: 8237-8248
作者:  Liang, Cui Y.;  Xu, Jing L.;  Xu, Hui J.;  Qi, Wei;  Zhang, Yu;  Luo, Wen;  Chen, Xiao Y.;  Wang, Zhong M.;  Yuan, Zhen H.
收藏  |  浏览/下载：227/0  |  提交时间：2020/10/29
beta-Glucosidase  Organic solvent stimulation  Succinic acid  Ethanol  Co-fermentation  
Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
收藏  |  浏览/下载：236/0  |  提交时间：2017/10/13
Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
作者:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
收藏  |  浏览/下载：142/0  |  提交时间：2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
收藏  |  浏览/下载：137/0  |  提交时间：2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization