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Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
作者:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
收藏  |  浏览/下载：447/0  |  提交时间：2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:  Tian, Miao;  Fu, Junying;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  He, Dong;  Li, Zhibing;  Liu, Tao;  Li, Ming;  Luo, Wen
收藏  |  浏览/下载：263/0  |  提交时间：2021/10/27
Rhizomucor miehei lipase  N-linked glycosylation site  Thermostability  Methanol tolerance  Biodiesel  
Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:  Zhang, Jun;  Chen, Xiaoyan;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
收藏  |  浏览/下载：298/0  |  提交时间：2021/10/27
Lipase  Bionic-immobilization  Biodiesel  Bio-silicification  Molecular dynamics simulation  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
作者:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
收藏  |  浏览/下载：339/0  |  提交时间：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil 期刊论文
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2021, 页码: 9
作者:  Zhang, Jun;  Chen, Huanjun;  Wang, Zhiyuan;  Xu, Huijuan;  Luo, Wen;  Xu, Jingliang;  Lv, Pengmei
收藏  |  浏览/下载：311/0  |  提交时间：2021/10/27
biodiesel  Escherichia coli  Lipase  
The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study 期刊论文
RSC ADVANCES, 2021, 卷号: 11, 期号: 25, 页码: 15054-15059
作者:  Song, Miaojia;  Di, Xiaohui;  Zhang, Yu;  Sun, Yongming;  Wang, Zhongming;  Yuan, Zhenhong;  Guo, Ying
收藏  |  浏览/下载：334/0  |  提交时间：2021/10/27