The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study

doi:10.1039/d1ra01780b

GIEC OpenIR

	The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study
	Song, Miaojia 1,3; Di, Xiaohui 2; Zhang, Yu1 ; Sun, Yongming1 ; Wang, Zhongming1 ; Yuan, Zhenhong1 ; Guo, Ying1
	2021-05-04
发表期刊	RSC ADVANCES
卷号	11 期号:25 页码:15054-15059
通讯作者	Zhang, Yu(zhangyu@ms.giec.ac.cn)
摘要	As an important bio-based chemical, methyl levulinate (ML) can be produced via enzymatic esterification of levulinic acid with methanol. A kinetic model is developed in this work based on the law of mass action and reaction reversibility, to investigate the effect of enzyme loading, alcohol/acid ratio and temperature on ML yield. Data analysis shows that newly developed binary regression is apparently more persuasive than the commonly used unitary regression. Kinetic study reveals: (1) rate constants of esterification/hydrolysis increase with increasing enzyme loading, while their ratio (equilibrium constant) remains invariant. (2) Methanol has no toxicity towards lipase, and hence, neither the rate constants of esterification/hydrolysis nor the equilibrium constant are affected by alcohol/acid ratio. (3) Both rate constants of esterification/hydrolysis and the equilibrium constant increase with temperature elevation, and their relationships agree with Arrhenius equation and Van't Hoff equation, respectively. (4) The esterification is endothermic and spontaneous. In total, the application of binary regression analysis for the developed model to study the enzymatic esterification kinetics is quite successful.
DOI	10.1039/d1ra01780b
关键词[WOS]	LIPASE-CATALYZED ESTERIFICATION ; CANDIDA-ANTARCTICA ; IMMOBILIZATION ; RESOLUTION
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21978288] ; China Scholarship Council[202008440326]
WOS研究方向	Chemistry
项目资助者	National Natural Science Foundation of China ; China Scholarship Council
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000648718400019
出版者	ROYAL SOC CHEMISTRY
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/33164
专题	中国科学院广州能源研究所
通讯作者	Zhang, Yu
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 2.Univ Strasbourg, CNRS UMR 7177, Strasbourg Inst Chem, Lab Synth Organ React & Catalysis, F-67000 Strasbourg, France 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Song, Miaojia,Di, Xiaohui,Zhang, Yu,et al. The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study[J]. RSC ADVANCES,2021,11(25):15054-15059.
APA	Song, Miaojia.,Di, Xiaohui.,Zhang, Yu.,Sun, Yongming.,Wang, Zhongming.,...&Guo, Ying.(2021).The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study.RSC ADVANCES,11(25),15054-15059.
MLA	Song, Miaojia,et al."The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study".RSC ADVANCES 11.25(2021):15054-15059.