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N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2022, 卷号: 160, 页码: 8
Authors:  Tian, Miao;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Liang, Cuiyi;  Li, Zhibing;  Yang, LingMei;  Liu, Tao;  Li, Ming;  Luo, Wen
Favorite  |  View/Download:198/0  |  Submit date:2023/11/21
N-glycosylation  Loop structure  Rhizomucor miehei lipase  Methanol tolerance  Biodiesel  
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
Authors:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
Favorite  |  View/Download:500/0  |  Submit date:2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
Authors:  Tian, Miao;  Fu, Junying;  Wang, Zhiyuan;  Miao, Changlin;  Lv, Pengmei;  He, Dong;  Li, Zhibing;  Liu, Tao;  Li, Ming;  Luo, Wen
Favorite  |  View/Download:322/0  |  Submit date:2021/10/27
Rhizomucor miehei lipase  N-linked glycosylation site  Thermostability  Methanol tolerance  Biodiesel  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
Authors:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
Favorite  |  View/Download:385/0  |  Submit date:2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Effect of Propeptide Variation on Properties of Rhizomucor miehei Lipase (vol 12, pg 330, 2018) 期刊论文
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY, 2018, 卷号: 12, 期号: 6, 页码: 572-572
Authors:  Luo, Wen;  He, Dong;  Fu, Junying;  Lv, Pengmei;  Yuan, Zhenhong;  Alam, Md. Asraful;  Wang, Zhiyuan;  Huang, Shaowei
Favorite  |  View/Download:218/0  |  Submit date:2020/10/29
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
Authors:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
Favorite  |  View/Download:186/0  |  Submit date:2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
Authors:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
Favorite  |  View/Download:159/0  |  Submit date:2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization