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Improved methanol tolerance of Rhizomucor miehei lipase based on N?glycosylation within the α-helix region and its application in biodiesel production 期刊论文
Biotechnology for Biofuels, 2021, 卷号: 14, 期号: 1
作者:  Tian,Miao;  Yang,Lingmei;  Wang,Zhiyuan;  Lv,Pengmei;  Fu,Junying;  Miao,Changlin;  Li,Ming;  Liu,Tao;  Luo,Wen
收藏  |  浏览/下载:154/0  |  提交时间:2022/09/20
Rhizomucor miehei lipase  α-Helix region  N?Glycosylation  Methanol tolerance  Biodiesel  
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
作者:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
收藏  |  浏览/下载:437/0  |  提交时间:2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
作者:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
收藏  |  浏览/下载:331/0  |  提交时间:2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
收藏  |  浏览/下载:261/0  |  提交时间:2017/10/13
Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
作者:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
收藏  |  浏览/下载:148/0  |  提交时间:2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
收藏  |  浏览/下载:140/0  |  提交时间:2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization  
米黑根毛霉脂肪酶基因的克隆、表达及活性分析 期刊论文
生物技术通报, 2013, 期号: 6, 页码: 122
作者:  苗长林;  罗文;  刘姝娜;  吕鹏梅;  李惠文;  杨玲梅;  袁振宏;  蒋剑春
收藏  |  浏览/下载:134/0  |  提交时间:2021/11/01
米黑根毛霉  脂肪酶  毕赤酵母  基因克隆  基因表达  
米黑根毛霉脂肪酶基因的克隆表达及活性分析 期刊论文
生物技术通报, 2013, 期号: 6, 页码: 122
作者:  苗长林;  罗文;  刘姝娜;  吕鹏梅;  李惠文;  杨玲梅;  袁振宏;  蒋剑春
收藏  |  浏览/下载:202/0  |  提交时间:2019/11/30