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Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
收藏  |  浏览/下载：236/0  |  提交时间：2017/10/13
Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass 期刊论文
BIORESOURCES, 2017, 卷号: 12, 期号: 1, 页码: 434-447
作者:  Wu, Hongli;  Xue, Yong;  Li, Hailong;  Gan, Lihui;  Liu, Jian;  Long, Minnan
收藏  |  浏览/下载：224/0  |  提交时间：2017/10/13
Aspergillus Niger  Acetyl Xylan Esterase  Pichia Pastoris  Characterization  Synergistic Cooperation  
Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass 期刊论文
BIORESOURCES, 2017, 卷号: 12, 期号: 1, 页码: 434-447
作者:  Wu, Hongli;  Xue, Yong;  Li, Hailong;  Gan, Lihui;  Liu, Jian;  Long, Minnan
收藏  |  浏览/下载：160/0  |  提交时间：2017/10/13
Aspergillus Niger  Acetyl Xylan Esterase  Pichia Pastoris  Characterization  Synergistic Cooperation  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
作者:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
收藏  |  浏览/下载：142/0  |  提交时间：2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
米黑根毛霉脂肪酶在毕赤酵母中的高效表达及酶学性质研究 期刊论文
林产化学与工业, 2016, 期号: 1, 页码: 135-140
作者:  罗文;  王治元;  苗长林;  吕鹏梅;  何东;  李惠文;  杨玲梅;  袁振宏
浏览  |  Adobe PDF(688Kb)  |  收藏  |  浏览/下载：449/104  |  提交时间：2017/12/31
米黑根毛霉脂肪酶  毕赤酵母  密码子优化  酶学性质  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
收藏  |  浏览/下载：137/0  |  提交时间：2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization