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Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
收藏  |  浏览/下载：260/0  |  提交时间：2017/10/13
Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass 期刊论文
BIORESOURCES, 2017, 卷号: 12, 期号: 1, 页码: 434-447
作者:  Wu, Hongli;  Xue, Yong;  Li, Hailong;  Gan, Lihui;  Liu, Jian;  Long, Minnan
收藏  |  浏览/下载：240/0  |  提交时间：2017/10/13
Aspergillus Niger  Acetyl Xylan Esterase  Pichia Pastoris  Characterization  Synergistic Cooperation  
Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass 期刊论文
BIORESOURCES, 2017, 卷号: 12, 期号: 1, 页码: 434-447
作者:  Wu, Hongli;  Xue, Yong;  Li, Hailong;  Gan, Lihui;  Liu, Jian;  Long, Minnan
收藏  |  浏览/下载：164/0  |  提交时间：2017/10/13
Aspergillus Niger  Acetyl Xylan Esterase  Pichia Pastoris  Characterization  Synergistic Cooperation  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
作者:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
收藏  |  浏览/下载：148/0  |  提交时间：2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
收藏  |  浏览/下载：140/0  |  提交时间：2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization