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Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
作者:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
收藏  |  浏览/下载:447/0  |  提交时间:2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
作者:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
收藏  |  浏览/下载:339/0  |  提交时间:2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
收藏  |  浏览/下载:266/0  |  提交时间:2017/10/13
Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
作者:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
收藏  |  浏览/下载:152/0  |  提交时间:2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
米黑根毛霉脂肪酶在毕赤酵母中的高效表达及酶学性质研究 期刊论文
林产化学与工业, 2016, 期号: 1, 页码: 135-140
作者:  罗文;  王治元;  苗长林;  吕鹏梅;  何东;  李惠文;  杨玲梅;  袁振宏
浏览  |  Adobe PDF(688Kb)  |  收藏  |  浏览/下载:483/116  |  提交时间:2017/12/31
米黑根毛霉脂肪酶  毕赤酵母  密码子优化  酶学性质  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
作者:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
收藏  |  浏览/下载:142/0  |  提交时间:2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization