Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100

doi:10.3109/10242422.2010.516391

GIEC OpenIR > 中国科学院广州能源研究所

	Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100
	Zhang, Yu1,2 ; Xu, Jing-Liang 1; Li, Dong 1; Yuan, Zhen-Hong1
	2010-12-01
发表期刊	BIOCATALYSIS AND BIOTRANSFORMATION
ISSN	1024-2422
卷号	28 期号:5-6 页码:313-319
产权排序	[Zhang, Yu; Xu, Jing-Liang; Li, Dong; Yuan, Zhen-Hong] Chinese Acad Sci, Guangzhou Inst Energy & Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Peoples R China; [Zhang, Yu] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
通讯作者	yuanzh@ms.giec.ac.cn
摘要	To prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix Eudragit L-100 by non-covalent and covalent methods. Covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of N-hydroxysuccinimide. The temperature optimum of the cellulase was not improved apparently by immobilization but the pH optimum increased from 4.75 to 5.25. Immobilized cellulase was more active than free cellulase above pH 5.0. Immobilized cellulase was more stable than free cellulase during storage at 4 degrees C, room temperature and 50 degrees C. K(m) values of immobilized and free cellulase were 85.55 and 73.84 g L(-1), respectively. About 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw.
文章类型	Article
其他摘要	To prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix Eudragit L-100 by non-covalent and covalent methods. Covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of N-hydroxysuccinimide. The temperature optimum of the cellulase was not improved apparently by immobilization but the pH optimum increased from 4.75 to 5.25. Immobilized cellulase was more active than free cellulase above pH 5.0. Immobilized cellulase was more stable than free cellulase during storage at 4 degrees C, room temperature and 50 degrees C. K(m) values of immobilized and free cellulase were 85.55 and 73.84 g L(-1), respectively. About 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw.
关键词	Immobilized Cellulase Eudragit L-100 N-hydroxysuccinimide Carbodiimide Coupling Smart Biocatalysis
学科领域	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3109/10242422.2010.516391
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
URL	查看原文
关键词[WOS]	XYLAN-DEGRADING ENZYMES ; BIOETHANOL PRODUCTION ; ENZYMATIC-HYDROLYSIS ; POLYMER ; ETHANOL ; FERMENTATION ; PRETREATMENT ; CARRAGEENAN ; IMPROVEMENT ; CHITOSAN
收录类别	SCI
语种	英语
项目资助者	Chinese Academy of Sciences [KSCX-YW-11-A3, KSCX2-YW-G-075-09, KGCX2-YW-335]; National High Technology Research and Development Program of China [2007AA05Z406]
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000285554000003
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/8462
专题	中国科学院广州能源研究所生物质能源生化转化实验室
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy & Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yu,Xu, Jing-Liang,Li, Dong,et al. Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2010,28(5-6):313-319.
APA	Zhang, Yu,Xu, Jing-Liang,Li, Dong,&Yuan, Zhen-Hong.(2010).Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100.BIOCATALYSIS AND BIOTRANSFORMATION,28(5-6),313-319.
MLA	Zhang, Yu,et al."Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100".BIOCATALYSIS AND BIOTRANSFORMATION 28.5-6(2010):313-319.

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