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Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100
Zhang, Yu1,2; Xu, Jing-Liang1; Li, Dong1; Yuan, Zhen-Hong1
2010-12-01
Source PublicationBIOCATALYSIS AND BIOTRANSFORMATION
ISSN1024-2422
Volume28Issue:5-6Pages:313-319
Contribution Rank[Zhang, Yu; Xu, Jing-Liang; Li, Dong; Yuan, Zhen-Hong] Chinese Acad Sci, Guangzhou Inst Energy & Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Peoples R China; [Zhang, Yu] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Corresponding Authoryuanzh@ms.giec.ac.cn
AbstractTo prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix Eudragit L-100 by non-covalent and covalent methods. Covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of N-hydroxysuccinimide. The temperature optimum of the cellulase was not improved apparently by immobilization but the pH optimum increased from 4.75 to 5.25. Immobilized cellulase was more active than free cellulase above pH 5.0. Immobilized cellulase was more stable than free cellulase during storage at 4 degrees C, room temperature and 50 degrees C. K(m) values of immobilized and free cellulase were 85.55 and 73.84 g L(-1), respectively. About 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw.
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Other AbstractTo prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix Eudragit L-100 by non-covalent and covalent methods. Covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of N-hydroxysuccinimide. The temperature optimum of the cellulase was not improved apparently by immobilization but the pH optimum increased from 4.75 to 5.25. Immobilized cellulase was more active than free cellulase above pH 5.0. Immobilized cellulase was more stable than free cellulase during storage at 4 degrees C, room temperature and 50 degrees C. K(m) values of immobilized and free cellulase were 85.55 and 73.84 g L(-1), respectively. About 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw.
KeywordImmobilized Cellulase Eudragit L-100 N-hydroxysuccinimide Carbodiimide Coupling Smart Biocatalysis
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.3109/10242422.2010.516391
WOS Subject ExtendedBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
URL查看原文
WOS KeywordXYLAN-DEGRADING ENZYMES ; BIOETHANOL PRODUCTION ; ENZYMATIC-HYDROLYSIS ; POLYMER ; ETHANOL ; FERMENTATION ; PRETREATMENT ; CARRAGEENAN ; IMPROVEMENT ; CHITOSAN
Indexed BySCI
Language英语
Funding OrganizationChinese Academy of Sciences [KSCX-YW-11-A3, KSCX2-YW-G-075-09, KGCX2-YW-335]; National High Technology Research and Development Program of China [2007AA05Z406]
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000285554000003
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Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/8462
Collection中国科学院广州能源研究所
生物质能源生化转化实验室
Affiliation1.Chinese Acad Sci, Guangzhou Inst Energy & Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yu,Xu, Jing-Liang,Li, Dong,et al. Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2010,28(5-6):313-319.
APA Zhang, Yu,Xu, Jing-Liang,Li, Dong,&Yuan, Zhen-Hong.(2010).Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100.BIOCATALYSIS AND BIOTRANSFORMATION,28(5-6),313-319.
MLA Zhang, Yu,et al."Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100".BIOCATALYSIS AND BIOTRANSFORMATION 28.5-6(2010):313-319.
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