GIEC OpenIR  > 中国科学院广州能源研究所
Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100
Zhang, Yu1,2; Xu, Jing-Liang1; Li, Dong1; Yuan, Zhen-Hong1
2010-12-01
发表期刊BIOCATALYSIS AND BIOTRANSFORMATION
ISSN1024-2422
卷号28期号:5-6页码:313-319
产权排序[Zhang, Yu; Xu, Jing-Liang; Li, Dong; Yuan, Zhen-Hong] Chinese Acad Sci, Guangzhou Inst Energy & Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Peoples R China; [Zhang, Yu] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
通讯作者yuanzh@ms.giec.ac.cn
摘要To prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix Eudragit L-100 by non-covalent and covalent methods. Covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of N-hydroxysuccinimide. The temperature optimum of the cellulase was not improved apparently by immobilization but the pH optimum increased from 4.75 to 5.25. Immobilized cellulase was more active than free cellulase above pH 5.0. Immobilized cellulase was more stable than free cellulase during storage at 4 degrees C, room temperature and 50 degrees C. K(m) values of immobilized and free cellulase were 85.55 and 73.84 g L(-1), respectively. About 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw.
文章类型Article
其他摘要To prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix Eudragit L-100 by non-covalent and covalent methods. Covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of N-hydroxysuccinimide. The temperature optimum of the cellulase was not improved apparently by immobilization but the pH optimum increased from 4.75 to 5.25. Immobilized cellulase was more active than free cellulase above pH 5.0. Immobilized cellulase was more stable than free cellulase during storage at 4 degrees C, room temperature and 50 degrees C. K(m) values of immobilized and free cellulase were 85.55 and 73.84 g L(-1), respectively. About 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw.
关键词Immobilized Cellulase Eudragit L-100 N-hydroxysuccinimide Carbodiimide Coupling Smart Biocatalysis
学科领域Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.3109/10242422.2010.516391
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
URL查看原文
关键词[WOS]XYLAN-DEGRADING ENZYMES ; BIOETHANOL PRODUCTION ; ENZYMATIC-HYDROLYSIS ; POLYMER ; ETHANOL ; FERMENTATION ; PRETREATMENT ; CARRAGEENAN ; IMPROVEMENT ; CHITOSAN
收录类别SCI
语种英语
项目资助者Chinese Academy of Sciences [KSCX-YW-11-A3, KSCX2-YW-G-075-09, KGCX2-YW-335]; National High Technology Research and Development Program of China [2007AA05Z406]
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号WOS:000285554000003
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/8462
专题中国科学院广州能源研究所
生物质能源生化转化实验室
作者单位1.Chinese Acad Sci, Guangzhou Inst Energy & Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yu,Xu, Jing-Liang,Li, Dong,et al. Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2010,28(5-6):313-319.
APA Zhang, Yu,Xu, Jing-Liang,Li, Dong,&Yuan, Zhen-Hong.(2010).Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100.BIOCATALYSIS AND BIOTRANSFORMATION,28(5-6),313-319.
MLA Zhang, Yu,et al."Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100".BIOCATALYSIS AND BIOTRANSFORMATION 28.5-6(2010):313-319.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Preparation and prop(370KB) 限制开放CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Yu]的文章
[Xu, Jing-Liang]的文章
[Li, Dong]的文章
百度学术
百度学术中相似的文章
[Zhang, Yu]的文章
[Xu, Jing-Liang]的文章
[Li, Dong]的文章
必应学术
必应学术中相似的文章
[Zhang, Yu]的文章
[Xu, Jing-Liang]的文章
[Li, Dong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。