Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production | |
Zhang, Jun1,2,4; Chen, Xiaoyan1,4; Lv, Pengmei1 ; Luo, Wen1; Wang, Zhiyuan1; Xu, Jingliang1,3; Wang, Zhongming1
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| 2021-11-15 | |
| Source Publication | FUEL
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| ISSN | 0016-2361 |
| Volume | 304Pages:11 |
| Corresponding Author | Lv, Pengmei(lvpm@ms.giec.ac.cn) |
| Abstract | In this study, bio-silicification was employed to develop a green and simple bionic-immobilization method for the immobilization of recombinant lipase. The results showed that under the optimized bionic-immobilization conditions, a maximum immobilization efficiency and an activity recovery of 97.71% and 89.62%, respectively, were obtained. Further, bionic-immobilization significantly improved the thermal stability, storage stability, and short-chain alcohol resistance of the bionic-immobilized lipase. Furthermore, molecular dynamics simulation of the bionic-immobilization mechanism revealed that the key amino acid residues that facilitated the binding of the recombinant lipase to silica particles included Arg336, Lys345, His391, His392, His394, and His395, indicating that hydrogen bonds had a special effect in intermolecular recognition, and the active center of the bionic-immobilized lipase favored substrate entry due to the conformational changes of Phe291 and Ile320. Finally, biodiesel was prepared using the bionic-immobilized lipase as catalyst, and after optimizing the enzymatic process, the biodiesel yield reached 91.04% using 20% immobilized lipase dosage within 12 h at 45 degrees C, and a catalytic activity of 55.02% was observed following the reutilization of the bionic-immobilized lipase seven times. These findings suggest that bionic-immobilization is a novel and rapid method to realize recombinant enzyme immobilization, and can provide economic and ecological support for the biocatalytic preparation of industrial products. |
| Keyword | Lipase Bionic-immobilization Biodiesel Bio-silicification Molecular dynamics simulation |
| DOI | 10.1016/j.fuel.2021.121594 |
| WOS Keyword | MOLECULAR-DYNAMICS SIMULATION ; ENZYME IMMOBILIZATION ; PROTEIN ; ADSORPTION ; MORPHOLOGY ; SUPPORT |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Program of China[2019YFB1504003] ; Projects of International Cooperation and Exchanges NSFC[51861145103] ; Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Program of CAS Key Laboratory of Renewable Energy[E029010801] ; Guangdong Special Support Program[2017TX04Z109] ; National Natural Science Foundation of China[51606201] |
| WOS Research Area | Energy & Fuels ; Engineering |
| Funding Organization | National Key Research and Development Program of China ; Projects of International Cooperation and Exchanges NSFC ; Natural Science Foundation of Guangdong Province of China ; Program of CAS Key Laboratory of Renewable Energy ; Guangdong Special Support Program ; National Natural Science Foundation of China |
| WOS Subject | Energy & Fuels ; Engineering, Chemical |
| WOS ID | WOS:000691213500004 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/33822 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Lv, Pengmei |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 2.Guangdong Ocean Univ, Coll Food Sci & Technol,Guangdong Prov Engn Lab M, Guangdong Prov Engn Technol Res Ctr Seafood, Key Lab Adv Proc Aquat Prod,Guangdong Higher Educ, Zhanjiang 524088, Peoples R China 3.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhang, Jun,Chen, Xiaoyan,Lv, Pengmei,et al. Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production[J]. FUEL,2021,304:11. |
| APA | Zhang, Jun.,Chen, Xiaoyan.,Lv, Pengmei.,Luo, Wen.,Wang, Zhiyuan.,...&Wang, Zhongming.(2021).Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production.FUEL,304,11. |
| MLA | Zhang, Jun,et al."Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production".FUEL 304(2021):11. |
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