Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22

doi:10.1007/s13205-017-1041-x

GIEC OpenIR

	Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22
	Long, Chuannan 1,2; Cui, Jingjing 1,3,4; Li, Hailong 3,5; Liu, Jian 3; Gan, Lihui 3; Zeng, Bin 1,2; Long, Minnan 3
	2017-12-20
发表期刊	3 BIOTECH
ISSN	2190-572X
卷号	8 页码:6
通讯作者	Long, Minnan(longmn@xmu.edu.cn)
摘要	The goal of this study was to enhance the production of xylooligosaccharides (XOs) and reduce the production of xylose. We investigated beta-xylosidases, which were key enzymes in the hydrolysis of xylan into xylose, in Trichoderma orientalis EU7-22. The binary vector pUR5750G/bxl::hph was constructed to knock out the beta-xyl1 gene (encoding beta-xylosidases) in T. orientalis EU7-22 by homologous integration, producing the mutant strain T. orientalis Bxyl-1. Xylanase activity for strain Bxyl-1 was 452.42 IU/mL, which increased by only 0.07% compared to that of parental strain EU7-22, whereas beta-xylosidase activity was 0.06 IU/mL, representing a 91.89% decrease. When xylanase (200 IU/g xylan), produced by T. orientalis EU7-22 and T. orientalis Bxyl-1, was used to hydrolyze beechwood xylan, in contrast to the parental strain, the XOs were enhanced by 83.27%, whereas xylose decreased by 45.80% after 36 h in T. orientalis Bxyl-1. Based on these results, T. orientalis Bxyl-1 has great potential for application in the production of XOs from lignocellulosic biomass.
关键词	Trichoderma orientalis Xylanase beta-xylosidase beta-xyl1 gene Xylooligosaccharides Xylose
DOI	10.1007/s13205-017-1041-x
关键词[WOS]	FILAMENTOUS FUNGI ; BETA-XYLOSIDASES ; HYPOCREA-ORIENTALIS ; ASPERGILLUS-NIGER ; XYLAN ; EXPRESSION ; PROGRESS ; XYLOSE ; REESEI ; BREAKS
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31170067] ; National Natural Science Foundation of China[31600475] ; National Natural Science Foundation of China[21303142] ; Jiangxi Province Science Foundation for Youths[20161BAB214177] ; Natural Science Foundation of Guangdong Province[2016A030310124]
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Natural Science Foundation of China ; Jiangxi Province Science Foundation for Youths ; Natural Science Foundation of Guangdong Province
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000418567500001
出版者	SPRINGER HEIDELBERG
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/22813
专题	中国科学院广州能源研究所
通讯作者	Long, Minnan
作者单位	1.Jiangxi Sci & Technol Normal Univ, Jiangxi Key Lab Bioproc Engn, Nanchang 330013, Jiangxi, Peoples R China 2.Jiangxi Sci & Technol Normal Univ, Sch Life Sci, Nanchang 330013, Jiangxi, Peoples R China 3.Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China 4.Xiamen Univ, Natl Engn Lab Green Chem Prod Alcohols Ethers Est, Xiamen 361005, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Long, Chuannan,Cui, Jingjing,Li, Hailong,et al. Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22[J]. 3 BIOTECH,2017,8:6.
APA	Long, Chuannan.,Cui, Jingjing.,Li, Hailong.,Liu, Jian.,Gan, Lihui.,...&Long, Minnan.(2017).Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22.3 BIOTECH,8,6.
MLA	Long, Chuannan,et al."Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22".3 BIOTECH 8(2017):6.