Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22 | |
| Long, Chuannan1,2; Cui, Jingjing1,3,4; Li, Hailong3,5; Liu, Jian3; Gan, Lihui3; Zeng, Bin1,2; Long, Minnan3 | |
| 2017-12-20 | |
| Source Publication | 3 BIOTECH
 |
| ISSN | 2190-572X |
| Volume | 8Pages:6 |
| Corresponding Author | Long, Minnan(longmn@xmu.edu.cn) |
| Abstract | The goal of this study was to enhance the production of xylooligosaccharides (XOs) and reduce the production of xylose. We investigated beta-xylosidases, which were key enzymes in the hydrolysis of xylan into xylose, in Trichoderma orientalis EU7-22. The binary vector pUR5750G/bxl::hph was constructed to knock out the beta-xyl1 gene (encoding beta-xylosidases) in T. orientalis EU7-22 by homologous integration, producing the mutant strain T. orientalis Bxyl-1. Xylanase activity for strain Bxyl-1 was 452.42 IU/mL, which increased by only 0.07% compared to that of parental strain EU7-22, whereas beta-xylosidase activity was 0.06 IU/mL, representing a 91.89% decrease. When xylanase (200 IU/g xylan), produced by T. orientalis EU7-22 and T. orientalis Bxyl-1, was used to hydrolyze beechwood xylan, in contrast to the parental strain, the XOs were enhanced by 83.27%, whereas xylose decreased by 45.80% after 36 h in T. orientalis Bxyl-1. Based on these results, T. orientalis Bxyl-1 has great potential for application in the production of XOs from lignocellulosic biomass. |
| Keyword | Trichoderma orientalis Xylanase beta-xylosidase beta-xyl1 gene Xylooligosaccharides Xylose |
| DOI | 10.1007/s13205-017-1041-x |
| WOS Keyword | FILAMENTOUS FUNGI ; BETA-XYLOSIDASES ; HYPOCREA-ORIENTALIS ; ASPERGILLUS-NIGER ; XYLAN ; EXPRESSION ; PROGRESS ; XYLOSE ; REESEI ; BREAKS |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31170067] ; National Natural Science Foundation of China[31600475] ; National Natural Science Foundation of China[21303142] ; Jiangxi Province Science Foundation for Youths[20161BAB214177] ; Natural Science Foundation of Guangdong Province[2016A030310124] |
| WOS Research Area | Biotechnology & Applied Microbiology |
| Funding Organization | National Natural Science Foundation of China ; Jiangxi Province Science Foundation for Youths ; Natural Science Foundation of Guangdong Province |
| WOS Subject | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000418567500001 |
| Publisher | SPRINGER HEIDELBERG |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/22813 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Long, Minnan |
| Affiliation | 1.Jiangxi Sci & Technol Normal Univ, Jiangxi Key Lab Bioproc Engn, Nanchang 330013, Jiangxi, Peoples R China 2.Jiangxi Sci & Technol Normal Univ, Sch Life Sci, Nanchang 330013, Jiangxi, Peoples R China 3.Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China 4.Xiamen Univ, Natl Engn Lab Green Chem Prod Alcohols Ethers Est, Xiamen 361005, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China |
| Recommended Citation GB/T 7714 | Long, Chuannan,Cui, Jingjing,Li, Hailong,et al. Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22[J]. 3 BIOTECH,2017,8:6. |
| APA | Long, Chuannan.,Cui, Jingjing.,Li, Hailong.,Liu, Jian.,Gan, Lihui.,...&Long, Minnan.(2017).Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22.3 BIOTECH,8,6. |
| MLA | Long, Chuannan,et al."Improvement in xylooligosaccharides production by knockout of the beta-xyl1 gene in Trichoderma orientalis EU7-22".3 BIOTECH 8(2017):6. |
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