木糖异构酶基因xylA表达载体构建

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	木糖异构酶基因xylA表达载体构建
	杨柳; 叶菁; 陈小燕; 许敬亮; 袁振宏
	2014
Source Publication	微生物学杂志
ISSN	1005-7021
Volume	034 Issue:003 Pages:9
Abstract	以大肠埃希菌 MG1655的基因组为模板，通过PCR扩增获得木糖异构酶基因xylA。利用敲除编码对基因转录起负调控作用的lacIq基因的大肠埃希菌/谷氨酸棒杆菌穿梭质粒pEC-XK99E，酶连后转化大肠埃希菌BL21和谷氨酸棒杆菌ATCC 13032。成功构建出了具有大肠埃希菌BL21表达活性的木糖异构酶表达载体Ptrc-xylA。
Language	英语
Document Type	期刊论文
Identifier	http://ir.giec.ac.cn/handle/344007/21569
Collection	中国科学院广州能源研究所
Affiliation	中国科学院广州能源研究所
First Author Affilication	GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation GB/T 7714	杨柳,叶菁,陈小燕,等. 木糖异构酶基因xylA表达载体构建[J]. 微生物学杂志,2014,034(003):9.
APA	杨柳,叶菁,陈小燕,许敬亮,&袁振宏.(2014).木糖异构酶基因xylA表达载体构建.微生物学杂志,034(003),9.
MLA	杨柳,et al."木糖异构酶基因xylA表达载体构建".微生物学杂志 034.003(2014):9.

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