GIEC OpenIR
巴夫杜氏藻β-肌动蛋白基因的克隆和分析
尚常花; 朱顺妮; 袁振宏; 王忠铭
2011
Source Publication安徽农业科学
ISSN0517-6611
Volume039Issue:028Pages:17172
Abstract目的获得巴夫杜氏藻β-肌动蛋白基因cDNA全长序列。方法以巴夫杜氏藻cDNA为模板,采用简并引物进行PCR扩增,获得533bp特异cDNA片段。在此基础上,设计特异引物,采用5’-Genome Walking和3’-RACE的方法,获得基因的5’-端DNA序列和3’-端cDNA序列,进而获得β-肌动蛋白基因cDNA全长序列。结果获得了巴夫杜氏藻β-肌动蛋白基因的特异cDNA片段、5’-端DNA和3’-端cDNA片段。经拼接后,扩增出全长cDNA。β-肌动蛋白基因cDNA全长1754bp,包括1137bp的开放读码框和617bp的3’-非翻译区序列。氨基酸序列相似性分析发现,巴夫杜氏藻β-肌动蛋白氨基酸序列与杜氏盐藻、莱茵衣藻等的同源性较高。系统发育分析表明,巴夫杜氏藻β-肌动蛋白与杜氏盐藻的相似性最高。结论首次获得了巴夫杜氏藻β-肌动蛋白基因cDNA全长序列,并发现巴夫杜氏藻β-肌动蛋白基因非常保守。
Language英语
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/19450
Collection中国科学院广州能源研究所
Affiliation中国科学院广州能源研究所
First Author AffilicationGuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation
GB/T 7714
尚常花,朱顺妮,袁振宏,等. 巴夫杜氏藻β-肌动蛋白基因的克隆和分析[J]. 安徽农业科学,2011,039(028):17172.
APA 尚常花,朱顺妮,袁振宏,&王忠铭.(2011).巴夫杜氏藻β-肌动蛋白基因的克隆和分析.安徽农业科学,039(028),17172.
MLA 尚常花,et al."巴夫杜氏藻β-肌动蛋白基因的克隆和分析".安徽农业科学 039.028(2011):17172.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[尚常花]'s Articles
[朱顺妮]'s Articles
[袁振宏]'s Articles
Baidu academic
Similar articles in Baidu academic
[尚常花]'s Articles
[朱顺妮]'s Articles
[袁振宏]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[尚常花]'s Articles
[朱顺妮]'s Articles
[袁振宏]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.