GIEC OpenIR  > 中国科学院广州能源研究所
Alternative TitleIsolation and identification of a diesel alkanes degrading bacterium
吕乾川1,2; 伍元东1,2; 周俊1,2; 贾红华1,2; 韦萍1; 郑涛3; 雍晓雨1,2
Source Publication生物加工过程
Abstract从加油站附近土壤取样,以不同的柴油高级烃为碳源、菌体生物量(OD600)为指示,分离筛选柴油高级烃降解菌株,同时应用高效液相色谱(HPLC)、气相色谱-质谱(GC-MS)分析其降解率以及降解产物。结果筛选到一株对二苯并噻吩、芳香烃类都具有一定降解效果的菌株B5,它对二苯并噻吩的降解率为63.0%,芳香烃类降解率为83.4%。该菌株能将直链烷烃(如正十二烷)的末端羧基化形成羧酸类物质,在实际应用中具有提高油品润滑性质的潜力。经16S rRNA基因鉴定,该菌株为一株红平红球菌(Rhodococcus erythropolis),并被命名为R.erythropolis B5。
Other AbstractA bacterium with the ability of diesel alkanesdegradation was isolated from the soil sample near gas station with different diesel alkanes as carbon sources and biomass density(OD600) as indicator.High performance liquid chromatography(HPLC) and gas chromatography-mass spectrometry(GC-MS) were used to analyzed the degradation efficiency and the degradation products of the bacterium. The isolated bacterial strain B5 could metabolize and degrade dibenzothiophene and aromatic hydrocarbons,with the degradation efficiency of 63. 0% and 83. 4% respectively. In addition,the alkyl group at the end of straight-chain alkanes can be transformed into carboxyl group by the strain through carboxylation,which potentially helps to enhance the lubricating performance of diesel. Finally,this strain was identified to be Rhodococcus erythropolis by sequencing the 16 S rRNA gene and was named as R. erythropolis B5.
Keyword柴油高级烃 直链烷烃 红平红球菌 降解菌 diesel alkanes straight-chain alkanes Rhodococcus erythropolis degrading bacterium
DOI10. 3969 /j. issn. 1672-3678. 2017. 04. 010
Citation statistics
Document Type期刊论文
Corresponding Author雍晓雨
Recommended Citation
GB/T 7714
吕乾川,伍元东,周俊,等. 一株柴油高级烃降解菌的筛选与鉴定[J]. 生物加工过程,2017,15(4):57-63.
APA 吕乾川.,伍元东.,周俊.,贾红华.,韦萍.,...&雍晓雨.(2017).一株柴油高级烃降解菌的筛选与鉴定.生物加工过程,15(4),57-63.
MLA 吕乾川,et al."一株柴油高级烃降解菌的筛选与鉴定".生物加工过程 15.4(2017):57-63.
Files in This Item: Download All
File Name/Size DocType Version Access License
一株柴油高级烃降解菌的筛选与鉴定.pdf(493KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[吕乾川]'s Articles
[伍元东]'s Articles
[周俊]'s Articles
Baidu academic
Similar articles in Baidu academic
[吕乾川]'s Articles
[伍元东]'s Articles
[周俊]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[吕乾川]'s Articles
[伍元东]'s Articles
[周俊]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 一株柴油高级烃降解菌的筛选与鉴定.pdf
Format: Adobe PDF
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.