Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Production of D-lactate from glucose using Klebsiella pneumoniae mutants | |
| Feng, Xinjun1,2; Jiang, Liqun2; Han, Xiaojuan1; Liu, Xiutao1,3; Zhao, Zhiqiang1,3; Liu, Huizhou1; Xian, Mo1; Zhao, Guang1 | |
| 2017-11-21 | |
| Source Publication | MICROBIAL CELL FACTORIES
 |
| ISSN | 1475-2859 |
| Volume | 16Pages:11 |
| Corresponding Author | Liu, Huizhou(liuhuizhou@qibebt.ac.cn) ; Xian, Mo(xianmo@qibebt.ac.cn) ; Zhao, Guang(zhaoguang@qibebt.ac.cn) |
| Abstract | Background: D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. Results: Based on the redirection of carbon towards by-product formation, the effects of single-gene and multiple-gene deletions in K. pneumoniae on D-lactate production from glucose via acetolactate synthase (budB), acetate kinase (ackA), and alcohol dehydrogenase (adhE) were tested. Klebsiella pneumoniae mutants had different production behaviours. The accumulation of the main by-products was decreased in the mutants. The triple mutant strain had the most powerful ability to produce optically pure D-lactate from glucose, and was tested with xylose and arabinose as carbon sources. Fed-batch fermentation was also carried out under various aeration rates, and the strain accumulated 125.1 g/L D-lactate with a yield of 0.91 g/g glucose at 2.5 vvm. Conclusions: Knocking out by-product synthesis genes had a remarkable influence on the production and yield of D-lactate. This study demonstrated, for the first time, that K. pneumoniae has great potential to convert monosaccharides into D-lactate. The results provide new insights for industrial production of D-lactate by K. pneumoniae. |
| Keyword | D-Lactate Acetolactate synthase Acetate kinase Alcohol dehydrogenase Klebsiella pneumoniae |
| DOI | 10.1186/s12934-017-0822-6 |
| WOS Keyword | D-LACTIC ACID ; PURE D-LACTATE ; ESCHERICHIA-COLI ; SIMULTANEOUS SACCHARIFICATION ; 1,3-PROPANEDIOL PRODUCTION ; 3-HYDROXYPROPIONIC ACID ; MICROAEROBIC CONDITIONS ; GLYCEROL METABOLISM ; POLY(LACTIC ACID) ; DEFICIENT MUTANT |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31670089] ; CAS[ZDRW-ZS-2016-3M] ; Taishan Scholars Climbing Program of Shandong[TSPD20150210] ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion[Y707kb1001] ; CAS Key Laboratory of Bio-based Materials[KLBM 2016008] |
| WOS Research Area | Biotechnology & Applied Microbiology |
| Funding Organization | National Natural Science Foundation of China ; CAS ; Taishan Scholars Climbing Program of Shandong ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion ; CAS Key Laboratory of Bio-based Materials |
| WOS Subject | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000416061800003 |
| Publisher | BIOMED CENTRAL LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/16473 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Liu, Huizhou; Xian, Mo; Zhao, Guang |
| Affiliation | 1.Chinese Acad Sci, CAS Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Feng, Xinjun,Jiang, Liqun,Han, Xiaojuan,et al. Production of D-lactate from glucose using Klebsiella pneumoniae mutants[J]. MICROBIAL CELL FACTORIES,2017,16:11. |
| APA | Feng, Xinjun.,Jiang, Liqun.,Han, Xiaojuan.,Liu, Xiutao.,Zhao, Zhiqiang.,...&Zhao, Guang.(2017).Production of D-lactate from glucose using Klebsiella pneumoniae mutants.MICROBIAL CELL FACTORIES,16,11. |
| MLA | Feng, Xinjun,et al."Production of D-lactate from glucose using Klebsiella pneumoniae mutants".MICROBIAL CELL FACTORIES 16(2017):11. |
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