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Production of D-lactate from glucose using Klebsiella pneumoniae mutants
Feng, Xinjun1,2; Jiang, Liqun2; Han, Xiaojuan1; Liu, Xiutao1,3; Zhao, Zhiqiang1,3; Liu, Huizhou1; Xian, Mo1; Zhao, Guang1
2017-11-21
Source PublicationMICROBIAL CELL FACTORIES
ISSN1475-2859
Volume16Pages:11
Corresponding AuthorLiu, Huizhou(liuhuizhou@qibebt.ac.cn) ; Xian, Mo(xianmo@qibebt.ac.cn) ; Zhao, Guang(zhaoguang@qibebt.ac.cn)
AbstractBackground: D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. Results: Based on the redirection of carbon towards by-product formation, the effects of single-gene and multiple-gene deletions in K. pneumoniae on D-lactate production from glucose via acetolactate synthase (budB), acetate kinase (ackA), and alcohol dehydrogenase (adhE) were tested. Klebsiella pneumoniae mutants had different production behaviours. The accumulation of the main by-products was decreased in the mutants. The triple mutant strain had the most powerful ability to produce optically pure D-lactate from glucose, and was tested with xylose and arabinose as carbon sources. Fed-batch fermentation was also carried out under various aeration rates, and the strain accumulated 125.1 g/L D-lactate with a yield of 0.91 g/g glucose at 2.5 vvm. Conclusions: Knocking out by-product synthesis genes had a remarkable influence on the production and yield of D-lactate. This study demonstrated, for the first time, that K. pneumoniae has great potential to convert monosaccharides into D-lactate. The results provide new insights for industrial production of D-lactate by K. pneumoniae.
KeywordD-Lactate Acetolactate synthase Acetate kinase Alcohol dehydrogenase Klebsiella pneumoniae
DOI10.1186/s12934-017-0822-6
WOS KeywordD-LACTIC ACID ; PURE D-LACTATE ; ESCHERICHIA-COLI ; SIMULTANEOUS SACCHARIFICATION ; 1,3-PROPANEDIOL PRODUCTION ; 3-HYDROXYPROPIONIC ACID ; MICROAEROBIC CONDITIONS ; GLYCEROL METABOLISM ; POLY(LACTIC ACID) ; DEFICIENT MUTANT
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31670089] ; CAS[ZDRW-ZS-2016-3M] ; Taishan Scholars Climbing Program of Shandong[TSPD20150210] ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion[Y707kb1001] ; CAS Key Laboratory of Bio-based Materials[KLBM 2016008]
WOS Research AreaBiotechnology & Applied Microbiology
Funding OrganizationNational Natural Science Foundation of China ; CAS ; Taishan Scholars Climbing Program of Shandong ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion ; CAS Key Laboratory of Bio-based Materials
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000416061800003
PublisherBIOMED CENTRAL LTD
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/16473
Collection中国科学院广州能源研究所
Corresponding AuthorLiu, Huizhou; Xian, Mo; Zhao, Guang
Affiliation1.Chinese Acad Sci, CAS Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationGuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Feng, Xinjun,Jiang, Liqun,Han, Xiaojuan,et al. Production of D-lactate from glucose using Klebsiella pneumoniae mutants[J]. MICROBIAL CELL FACTORIES,2017,16:11.
APA Feng, Xinjun.,Jiang, Liqun.,Han, Xiaojuan.,Liu, Xiutao.,Zhao, Zhiqiang.,...&Zhao, Guang.(2017).Production of D-lactate from glucose using Klebsiella pneumoniae mutants.MICROBIAL CELL FACTORIES,16,11.
MLA Feng, Xinjun,et al."Production of D-lactate from glucose using Klebsiella pneumoniae mutants".MICROBIAL CELL FACTORIES 16(2017):11.
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