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在大肠杆菌中表达酮酸脱羧酶产异戊醇
Alternative TitleProduction of the Isoamyl Alcohol in E.coli by Expression of kivD Gene
肖仕圆1,2; 许敬亮1; 陈小燕1; 杨柳1; 袁振宏1
2015
Source Publication中国生物工程杂志
Issue4Pages:60-65
AbstractKeto acid decarboxylase is the key enzyme for biological synthesis of isoamyl alcohol, rarely existed in E. coli. Keto acid decarboxylase gene kivD(rbs) obtained from Lactococcuslactis subsp.Lactis genome DNA through reaction of PCR, was inserted into E.
Other Abstract酮酸脱羧酶作为异戊醇生物合成的关键酶,不存在于大肠杆菌中。以乳酸乳球菌的基因组DNA为模板,经过PCR扩增得到酮酸脱羧酶基因kivD(rbs),插入到大肠杆菌高效表达载体pET-28a(+)上形成pET-kivD(rbs),重组质粒热击转化进大肠杆菌 BL21(DE3) 中,其成功表达了酮酸脱羧酶。对发酵产物进行分析,检测到了微量的目标产物—异戊醇。
Keyword酮酸脱羧酶基因 重组质粒 转化 异戊醇
Funding Organization国家自然科学基金(21176237, 21211140237);国家"863"计划(2013AA065803);广东省科技攻关项目(2013B010403021);广州市科技攻关项目(2013J4300026);中国科学院院地合作项目;中国科学院广州能源研究所所长创新基金培育项目(y407p11001)
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/16269
Collection中国科学院广州能源研究所
生物质能源生化转化实验室
Affiliation1.中国科学院广州能源研究所中国科学院可再生能源与天然气水合物重点实验室,广州510640
2.中国科学院大学,北京100049
First Author AffilicationGuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation
GB/T 7714
肖仕圆,许敬亮,陈小燕,等. 在大肠杆菌中表达酮酸脱羧酶产异戊醇[J]. 中国生物工程杂志,2015(4):60-65.
APA 肖仕圆,许敬亮,陈小燕,杨柳,&袁振宏.(2015).在大肠杆菌中表达酮酸脱羧酶产异戊醇.中国生物工程杂志(4),60-65.
MLA 肖仕圆,et al."在大肠杆菌中表达酮酸脱羧酶产异戊醇".中国生物工程杂志 .4(2015):60-65.
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