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皮状丝孢酵母脂肪酸CoA连接酶(Facl)基因的克隆与分析
Alternative TitleCloning and Characterization of a Gene Encoding Fatty Acid CoA Ligase (Facl) from Trichosporon Cutaneum
尚常花; 王忠铭; 袁振宏; 朱顺妮; 秦磊; 吕鹏梅
2015
Source Publication中国粮油学报
Issue1Pages:71-75
AbstractThe Facl gene and its 5 '-flanking sequence has cloned from Trichosporon cutaneum and amalysis.Based on the former result of genome library sequencing, 898 bp DNA DNA sequence, the 5' genomic DNA was cloned by genome walking m were amplified respectively
Other Abstract从皮状丝孢酵母(Trichosporon cutaneum)中克隆了Facl基因及其5 '-上游序列,并对Facl基因及其5'-上游序列进行了分析.根据前期的基因组文库测序结果,获得了898 bp DNA序列.以此为基础,2次使用染色体步移(Genome walking)方法,分别获得了854 bp和1 236 bp 5'-DNA序列.根据获得的5和3端序列,合成引物用于扩增Facl基因的全长DNA和cDNA序列.分析发现,皮状丝孢酵母Facl基因cDNA包含一个1 662 bp的开放读码框,DNA不含内
Keyword皮状丝孢酵母 Facl基因 5'-上游序列 序列分析
Funding Organization国家科技支撑计划项目(2011BAD14803);国家自然科学基金项目(31100189)
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/16267
Collection中国科学院广州能源研究所
生物质能源生化转化实验室
Affiliation中国科学院可再生能源重点实验室中国科学院广州能源研究所,广州510640
First Author AffilicationGuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation
GB/T 7714
尚常花,王忠铭,袁振宏,等. 皮状丝孢酵母脂肪酸CoA连接酶(Facl)基因的克隆与分析[J]. 中国粮油学报,2015(1):71-75.
APA 尚常花,王忠铭,袁振宏,朱顺妮,秦磊,&吕鹏梅.(2015).皮状丝孢酵母脂肪酸CoA连接酶(Facl)基因的克隆与分析.中国粮油学报(1),71-75.
MLA 尚常花,et al."皮状丝孢酵母脂肪酸CoA连接酶(Facl)基因的克隆与分析".中国粮油学报 .1(2015):71-75.
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