Proteome response of Dunaliella parva induced by nitrogen limitation

doi:10.1016/j.algal.2017.01.016

GIEC OpenIR > 中国科学院广州能源研究所

	Proteome response of Dunaliella parva induced by nitrogen limitation
	Shang, Changhua1 ; Zhu, Shunni1 ; Wang, Zhongming1 ; Qin, Lei1 ; Alam, Mohammad Asraful 1; Xie, Jun 3,4; Yuan, Zhenhong1,2
	2017-04-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷号	23 页码:196-202
摘要	Nitrogen limitation is the most widely used method for directing metabolic flux to lipid biosynthesis in microalgae. Protein level changes induced by environmental stress could be investigated by proteomics analysis, which was a favourable approach for understanding the induction mechanisms. Although, we previously provided the results of transcriptome sequencing of Dunaliella parva under nitrogen limitation stress, the effects of nitrogen limitation on the microalgal proteome were not reported. The impact of nitrogen limitation on the proteome of green alga D. parva was investigated using iTRAQ quantitative proteomics technique. A total of 227 proteinswere up-regulated, and 159 proteinswere down-regulated under the nitrogen limitation condition. Functional analyses of differentially expressed proteins revealed that nitrogen limitation could induce protein level changes related to photosynthesis, stress response, lipid metabolism, carbohydrate metabolism and nitrogen metabolism. This study, together with our previous transcriptome sequencing of D. parva, therefore provides a foundation for understanding the regulatory mechanism of lipid biosynthesis induced by nitrogen limitation, which could further guide the genetic modification of lipid accumulation in D. parva. (C) 2016 Published by Elsevier B. V.
文章类型	Article
关键词	Dunaliella Parva Nitrogen Limitation Lipid Production Proteome Itraq Differential Expression
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.algal.2017.01.016
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	DIATOM PHAEODACTYLUM-TRICORNUTUM ; CHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; NANNOCHLOROPSIS-OCEANICA ; CARBON METABOLISM ; GREEN-ALGAE ; FATTY-ACIDS ; STRESS ; DEPRIVATION ; MECHANISMS
收录类别	SCI
语种	英语
项目资助者	Natural Science Foundation of Guangdong Province(2016A030313171 ; National Key Research and Development Program-China(2016YFB0601004) ; Pearl River S&T Nova Program of Guangzhou, China(201610010155) ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences(y507j21001 ; 2016A030312007) ; y507jb1001)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000397462700022
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/14107
专题	中国科学院广州能源研究所
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangdong Key Lab New & Renewable Energy Res & De, Key Lab Renewable Energy, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China 2.Collaborat Innovat Ctr Biomass Energy, 63 Nongye Rd, Zhengzhou 450002, Henan, Peoples R China 3.South China Agr Univ, Key Lab Energy Plants Resource & Utilizat, Minist Agr, Inst New Energy & New Mat, Guangzhou, Guangdong, Peoples R China 4.Guangdong Regular Higher Educ Inst, Key Lab Biomass Energy, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Shang, Changhua,Zhu, Shunni,Wang, Zhongming,et al. Proteome response of Dunaliella parva induced by nitrogen limitation[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2017,23:196-202.
APA	Shang, Changhua.,Zhu, Shunni.,Wang, Zhongming.,Qin, Lei.,Alam, Mohammad Asraful.,...&Yuan, Zhenhong.(2017).Proteome response of Dunaliella parva induced by nitrogen limitation.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,23,196-202.
MLA	Shang, Changhua,et al."Proteome response of Dunaliella parva induced by nitrogen limitation".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 23(2017):196-202.