Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva | |
| Shang, Changhua1,2,3,4; Xu, Xiaoli1,2,3; Yuan, Zhenhong4; Wang, Zhongming4; Hu, Lei5; Alam, Mohammad Asraful4; Xie, Jun1,2,3 | |
| 2016-08-01 | |
| Source Publication | JOURNAL OF APPLIED PHYCOLOGY
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| Volume | 28Issue:4Pages:2397-2405 |
| Abstract | Dunaliella parva can thrive and adapt to salt stress over a wide range of NaCl concentrations which also is related to carotenoid accumulation in Dunaliella. Dunaliella parva can accumulate carotenoids; however, the underlying mechanism of carotenoid accumulation needs further research. Thus, it is necessary to study biosynthesis and regulation of carotenoids for understanding carotenoid accumulation. In the present study, a gene encoding geranylgeranyl diphosphate synthase (GGPS) from the halophilic green alga D. parva has been cloned and analyzed. It is in the branch of terpene metabolism and named as DpGGPS (D. parva GGPS). The full-length complementary DNA (cDNA) of DpGGPS was 1612 bp, including an open reading frame (ORF) of 1059 bp, 189 bp 5'-untranslated region (5'-UTR) and 364 bp 3'-UTR. The 5'-flanking region was obtained by the genome walking method. Potential regulatory elements, associated with hormones, defense, and stress, were found in the 1310 bp 5'-flanking region. Functional characterization of DpGGPS in E. coli BL21(DE3) demonstrated that DpGGPS encoded a functional GGPS. Analysis of DpGGPS expression revealed a correlation between GGPS expression and the shift of NaCl concentration, which indicated that GGPS could be a salt stress responsive gene. Cloning and expression analysis of GGPS provides a foundation for studying the regulatory mechanism of carotenoid biosynthesis, adaptation mechanism to salt stress, and massive accumulation of carotenoids in D. parva. |
| Subtype | Article |
| Keyword | Dunaliella Parva Salt Stress Carotenoid Geranylgeranyl Diphosphate Synthase Complementation Analysis Differential Expression |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s10811-015-0767-2 |
| WOS Subject Extended | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS Keyword | BETA-CAROTENE ; FUNCTIONAL-ANALYSIS ; SALINA ; BIOSYNTHESIS ; METABOLISM ; EMPHASIS ; PLANTS ; ALGAE ; LIGHT |
| Indexed By | SCI |
| Language | 英语 |
| Funding Organization | National Natural Sciences Foundation of China(51476177) ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences(y507j21001) ; Foundation of Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology (Huaiyin Normal University)(JSBEET1316) |
| WOS Subject | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS ID | WOS:000383571300027 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/13931 |
| Collection | 中国科学院广州能源研究所 |
| Affiliation | 1.South China Agr Univ, Inst New Energy & New Mat, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China 2.Minist Agr, Key Lab Energy Plants Resource & Utilizat, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China 3.Key Lab Biomass Energy Guangdong Regular Higher E, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China 5.Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, 111 West Changjiang Rd, Huaian 223300, Jiangsu, Peoples R China |
| Recommended Citation GB/T 7714 | Shang, Changhua,Xu, Xiaoli,Yuan, Zhenhong,et al. Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(4):2397-2405. |
| APA | Shang, Changhua.,Xu, Xiaoli.,Yuan, Zhenhong.,Wang, Zhongming.,Hu, Lei.,...&Xie, Jun.(2016).Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva.JOURNAL OF APPLIED PHYCOLOGY,28(4),2397-2405. |
| MLA | Shang, Changhua,et al."Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva".JOURNAL OF APPLIED PHYCOLOGY 28.4(2016):2397-2405. |
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