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Discovery of genes for production of biofuels through transcriptome sequencing of Dunaliella parva
Shang, Changhua1,2,3,4,5,6; Bi, Guican1,2,3,6; Yuan, Zhenhong4,7; Wang, Zhongming4,7; Alam, Mohammad Asraful4,7; Xie, Jun1,2,3,6
2016
发表期刊ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷号13页码:318-326
摘要Compared with first generation plant-based biofuels, microalgae have potential advantages as feedstock for biofuel production. However, the genome sequences were insufficient in non-model microalgae with potential for biofuel production, which limited the development of genetic engineering of these microalgae. Here we described the de novo transcriptome sequencing and assembly for the halophilic green alga Dunaliella parva and identified important pathways, genes and transcription factor gene wri1 involved in biofuel production. The Illumina Hiseq 2000 platform was applied to D. parva transcriptome sequencing, which produced 26,304,060 (assembled into 60,883 contigs) and 26,797,446 (assembled into 55,236 contigs) high quality reads in samples SCH-5.0mMA (control sample) and SCH-0.5mMA (nitrogen limitation sample) respectively. Assembled contigs were subjected to Blastx search and annotated with NCBI non-redundant protein database, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes orthology identifiers. These analyses obtained the majority of genes involved in the biosynthesis and degradation of lipid and starch in D. parva. In addition, we have identified numerous differentially expressed genes, especially important transcription factor gene wri1 (GenBank KR185335) and studied differential expression of wri1 between control and nitrogen limitation sample, which provided foundation for illuminating the regulation mechanism of lipid biosynthesis induced by nitrogen limitation. (C) 2015 Elsevier B.V. All rights reserved.
文章类型Article
关键词Dunaliella Parva Transcriptome Sequencing Nitrogen Limitation Biofuel Production Transcription Factor Wrinkled1 Differential Expression
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.algal.2015.12.012
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]ACETYL-COA CARBOXYLASE ; CHLAMYDOMONAS-REINHARDTII ; RNA-SEQ ; ANNOTATION ; PATHWAY ; ARABIDOPSIS ; BIOSYNTHESIS ; ACCUMULATION ; WRINKLED1 ; GENOME
收录类别SCI
语种英语
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000370183100035
引用统计
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/11119
专题中国科学院广州能源研究所
作者单位1.South China Agr Univ, Inst New Energy & New Mat, Guangzhou, Guangdong, Peoples R China
2.Minist Agr, Key Lab Energy Plants Resource & Utilizat, Beijing, Peoples R China
3.Guangdong Regular Higher Educ Inst, Key Lab Biomass Energy, Guangzhou 510642, Guangdong, Peoples R China
4.Guangzhou Inst Energy Convers, Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
5.Jiangsu Key Lab Biomass Based Green Fuels & Chem, Nanjing 210037, Jiangsu, Peoples R China
6.483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
7.2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China
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Shang, Changhua,Bi, Guican,Yuan, Zhenhong,et al. Discovery of genes for production of biofuels through transcriptome sequencing of Dunaliella parva[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2016,13:318-326.
APA Shang, Changhua,Bi, Guican,Yuan, Zhenhong,Wang, Zhongming,Alam, Mohammad Asraful,&Xie, Jun.(2016).Discovery of genes for production of biofuels through transcriptome sequencing of Dunaliella parva.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,13,318-326.
MLA Shang, Changhua,et al."Discovery of genes for production of biofuels through transcriptome sequencing of Dunaliella parva".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 13(2016):318-326.
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