Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases | |
| Li, Hailong1,2; Xue, Yong1; Wu, Jinlian1; Wu, Hongli1; Qin, Guanghui1; Li, Chengguo1; Ding, Jiajia1; Liu, Jian1; Gan, Lihui1; Long, Minnan1 | |
| 2016 | |
| Source Publication | INDUSTRIAL CROPS AND PRODUCTS
 |
| Volume | 79Pages:170-179 |
| Abstract | Hemicelluloses isolated from holocellulose (MHOC) from Miscanthus were characterized by RP-HPLC-UV, FT-IR, and NMR. The hemicelluloses and recombinant hemicellulases, including endo-beta-1,4-xylanases (HoXyn11A and AnXyn10C), beta-xylosidases (AnXln3D), and alpha-L-arabinofuranosidases (AnAxh62A), as well as their interaction mechanisms were investigated by enzymatic hydrolysis. AnXyn10C released shorter end products than HoXyn11A from isolated hemicelluloses (IHEC). AnAxh62A was able to release all single-substituted alpha-L-arabinofuranosyl residues from IHEC. AnXynl10C and HoXyn11A were able to directly act on MHOC, whereas AnAxh62A and AnXln3D did not. The combination of HoXyn11A and AnAxh62A produced the highest xylo-oligosaccharides (XOS) yield from IHEC, whereas AnXynl10C alone produced the highest XOS yield from MHOC. The combination of HoXyn11A, AnAxh62A, and AnXln3D achieved the highest xylose yield from IHEC, whereas the combination of AnXyn10C, AnAxh62A, and AnXln3D achieved the highest xylose yield from MHOC. This study contributes to the development of efficient enzyme cocktails for the bioconversion of hemicelluloses from Miscanthus into monosaccharides and XOS. (C) 2015 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Hemicelluloses Miscanthus Monosaccharides Recombinant Hemicellulases Xylo-oligosaccharides |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.indcrop.2015.11.021 |
| WOS Subject Extended | Agriculture |
| WOS Keyword | GRADED ETHANOL PRECIPITATION ; SWITCHGRASS PANICUM-VIRGATUM ; THERMOASCUS-AURANTIACUS ; SUGARCANE BAGASSE ; X-GIGANTEUS ; L. XYLAN ; DEGRADATION ; ENZYMES ; BIOMASS ; DELIGNIFICATION |
| Indexed By | SCI |
| Language | 英语 |
| WOS Subject | Agricultural Engineering ; Agronomy |
| WOS ID | WOS:000367857300021 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/10948 |
| Collection | 中国科学院广州能源研究所 |
| Affiliation | 1.Xiamen Univ, Coll Energy, Xiamen 361102, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Hailong,Xue, Yong,Wu, Jinlian,et al. Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases[J]. INDUSTRIAL CROPS AND PRODUCTS,2016,79:170-179. |
| APA | Li, Hailong.,Xue, Yong.,Wu, Jinlian.,Wu, Hongli.,Qin, Guanghui.,...&Long, Minnan.(2016).Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases.INDUSTRIAL CROPS AND PRODUCTS,79,170-179. |
| MLA | Li, Hailong,et al."Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases".INDUSTRIAL CROPS AND PRODUCTS 79(2016):170-179. |
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