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Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases
Li, Hailong1,2; Xue, Yong1; Wu, Jinlian1; Wu, Hongli1; Qin, Guanghui1; Li, Chengguo1; Ding, Jiajia1; Liu, Jian1; Gan, Lihui1; Long, Minnan1
2016
发表期刊INDUSTRIAL CROPS AND PRODUCTS
卷号79页码:170-179
摘要Hemicelluloses isolated from holocellulose (MHOC) from Miscanthus were characterized by RP-HPLC-UV, FT-IR, and NMR. The hemicelluloses and recombinant hemicellulases, including endo-beta-1,4-xylanases (HoXyn11A and AnXyn10C), beta-xylosidases (AnXln3D), and alpha-L-arabinofuranosidases (AnAxh62A), as well as their interaction mechanisms were investigated by enzymatic hydrolysis. AnXyn10C released shorter end products than HoXyn11A from isolated hemicelluloses (IHEC). AnAxh62A was able to release all single-substituted alpha-L-arabinofuranosyl residues from IHEC. AnXynl10C and HoXyn11A were able to directly act on MHOC, whereas AnAxh62A and AnXln3D did not. The combination of HoXyn11A and AnAxh62A produced the highest xylo-oligosaccharides (XOS) yield from IHEC, whereas AnXynl10C alone produced the highest XOS yield from MHOC. The combination of HoXyn11A, AnAxh62A, and AnXln3D achieved the highest xylose yield from IHEC, whereas the combination of AnXyn10C, AnAxh62A, and AnXln3D achieved the highest xylose yield from MHOC. This study contributes to the development of efficient enzyme cocktails for the bioconversion of hemicelluloses from Miscanthus into monosaccharides and XOS. (C) 2015 Elsevier B.V. All rights reserved.
文章类型Article
关键词Hemicelluloses Miscanthus Monosaccharides Recombinant Hemicellulases Xylo-oligosaccharides
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.indcrop.2015.11.021
研究领域[WOS]Agriculture
关键词[WOS]GRADED ETHANOL PRECIPITATION ; SWITCHGRASS PANICUM-VIRGATUM ; THERMOASCUS-AURANTIACUS ; SUGARCANE BAGASSE ; X-GIGANTEUS ; L. XYLAN ; DEGRADATION ; ENZYMES ; BIOMASS ; DELIGNIFICATION
收录类别SCI
语种英语
WOS类目Agricultural Engineering ; Agronomy
WOS记录号WOS:000367857300021
引用统计
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/10948
专题中国科学院广州能源研究所
作者单位1.Xiamen Univ, Coll Energy, Xiamen 361102, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
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Li, Hailong,Xue, Yong,Wu, Jinlian,et al. Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases[J]. INDUSTRIAL CROPS AND PRODUCTS,2016,79:170-179.
APA Li, Hailong.,Xue, Yong.,Wu, Jinlian.,Wu, Hongli.,Qin, Guanghui.,...&Long, Minnan.(2016).Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases.INDUSTRIAL CROPS AND PRODUCTS,79,170-179.
MLA Li, Hailong,et al."Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases".INDUSTRIAL CROPS AND PRODUCTS 79(2016):170-179.
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