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Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases
Li, Hailong1,2; Xue, Yong1; Wu, Jinlian1; Wu, Hongli1; Qin, Guanghui1; Li, Chengguo1; Ding, Jiajia1; Liu, Jian1; Gan, Lihui1; Long, Minnan1
2016
Source PublicationINDUSTRIAL CROPS AND PRODUCTS
Volume79Pages:170-179
AbstractHemicelluloses isolated from holocellulose (MHOC) from Miscanthus were characterized by RP-HPLC-UV, FT-IR, and NMR. The hemicelluloses and recombinant hemicellulases, including endo-beta-1,4-xylanases (HoXyn11A and AnXyn10C), beta-xylosidases (AnXln3D), and alpha-L-arabinofuranosidases (AnAxh62A), as well as their interaction mechanisms were investigated by enzymatic hydrolysis. AnXyn10C released shorter end products than HoXyn11A from isolated hemicelluloses (IHEC). AnAxh62A was able to release all single-substituted alpha-L-arabinofuranosyl residues from IHEC. AnXynl10C and HoXyn11A were able to directly act on MHOC, whereas AnAxh62A and AnXln3D did not. The combination of HoXyn11A and AnAxh62A produced the highest xylo-oligosaccharides (XOS) yield from IHEC, whereas AnXynl10C alone produced the highest XOS yield from MHOC. The combination of HoXyn11A, AnAxh62A, and AnXln3D achieved the highest xylose yield from IHEC, whereas the combination of AnXyn10C, AnAxh62A, and AnXln3D achieved the highest xylose yield from MHOC. This study contributes to the development of efficient enzyme cocktails for the bioconversion of hemicelluloses from Miscanthus into monosaccharides and XOS. (C) 2015 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordHemicelluloses Miscanthus Monosaccharides Recombinant Hemicellulases Xylo-oligosaccharides
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.indcrop.2015.11.021
WOS Subject ExtendedAgriculture
WOS KeywordGRADED ETHANOL PRECIPITATION ; SWITCHGRASS PANICUM-VIRGATUM ; THERMOASCUS-AURANTIACUS ; SUGARCANE BAGASSE ; X-GIGANTEUS ; L. XYLAN ; DEGRADATION ; ENZYMES ; BIOMASS ; DELIGNIFICATION
Indexed BySCI
Language英语
WOS SubjectAgricultural Engineering ; Agronomy
WOS IDWOS:000367857300021
Citation statistics
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10948
Collection中国科学院广州能源研究所
Affiliation1.Xiamen Univ, Coll Energy, Xiamen 361102, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Li, Hailong,Xue, Yong,Wu, Jinlian,et al. Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases[J]. INDUSTRIAL CROPS AND PRODUCTS,2016,79:170-179.
APA Li, Hailong.,Xue, Yong.,Wu, Jinlian.,Wu, Hongli.,Qin, Guanghui.,...&Long, Minnan.(2016).Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases.INDUSTRIAL CROPS AND PRODUCTS,79,170-179.
MLA Li, Hailong,et al."Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases".INDUSTRIAL CROPS AND PRODUCTS 79(2016):170-179.
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