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Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli
Chen, Xiaoyan; Xu, Jingliang; Yang, Liu; Yuan, Zhenhong; Xiao, Shiyuan; Zhang, Yu; Liang, Cuiyi; He, Minchao; Guo, Ying
2015-11-01
Source PublicationJOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume42Issue:11Pages:1473-1479
AbstractHigher alcohols, longer chain alcohols, contain more than 3 carbon atoms, showed close energy advantages as gasoline, and were considered as the next generation substitution for chemical fuels. Higher alcohol biosynthesis by native microorganisms mainly needs gene expression of heterologous keto acid decarboxylase and alcohol dehydrogenases. In the present study, branched-chain alpha-keto acid decarboxylase gene from Lactococcus lactis subsp. lactis CICC 6246 (Kivd) and alcohol dehydrogenases gene from Zymomonas mobilis CICC 41465 (AdhB) were transformed into Escherichia coli for higher alcohol production. SDS-PAGE results showed these two proteins were expressed in the recombinant strains. The resulting strain was incubated in LB medium at 37 A degrees C in Erlenmeyer flasks and much more 3-methyl-1-butanol (104 mg/L) than isobutanol (24 mg/L) was produced. However, in 5 g/L glucose-containing medium, the production of two alcohols was similar, 156 and 161 mg/L for C4 (isobutanol) and C5 (3-methyl-1-butanol) alcohol, respectively. Effects of fermentation factors including temperature, glucose content, and alpha-keto acid on alcohol production were also investigated. The increase of glucose content and the adding of alpha-keto acids facilitated the production of C4 and C5 alcohols. The enzyme activities of pure Kivd on alpha-ketoisovalerate and alpha-ketoisocaproate were 26.77 and 21.24 mu mol min(-1) mg(-1), respectively. Due to its ability on decarboxylation of alpha-ketoisovalerate and alpha-ketoisocaproate, the recombinant E. coli strain showed potential application on isoamyl alcohol and isobutanol production.
SubtypeArticle
Keyword3-methyl-1-butanol Isobutanol Alpha-keto Acid Decarboxylase Alcohol Dehydrogenase
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10295-015-1656-z
WOS Subject ExtendedBiotechnology & Applied Microbiology
WOS KeywordKETO ACID DECARBOXYLASE ; SACCHAROMYCES-CEREVISIAE ; ISOBUTANOL PRODUCTION ; LACTOCOCCUS-LACTIS ; PHENYLPYRUVATE DECARBOXYLASE ; CORYNEBACTERIUM-GLUTAMICUM ; BIOSYNTHESIS ; METABOLISM ; ENZYME ; IDENTIFICATION
Indexed BySCI
Language英语
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000362964900005
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10873
Collection中国科学院广州能源研究所
AffiliationChinese Acad Sci, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Chen, Xiaoyan,Xu, Jingliang,Yang, Liu,et al. Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(11):1473-1479.
APA Chen, Xiaoyan.,Xu, Jingliang.,Yang, Liu.,Yuan, Zhenhong.,Xiao, Shiyuan.,...&Guo, Ying.(2015).Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(11),1473-1479.
MLA Chen, Xiaoyan,et al."Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.11(2015):1473-1479.
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