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Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli
Chen, Xiaoyan; Xu, Jingliang; Yang, Liu; Yuan, Zhenhong; Xiao, Shiyuan; Zhang, Yu; Liang, Cuiyi; He, Minchao; Guo, Ying
2015-11-01
发表期刊JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷号42期号:11页码:1473-1479
摘要Higher alcohols, longer chain alcohols, contain more than 3 carbon atoms, showed close energy advantages as gasoline, and were considered as the next generation substitution for chemical fuels. Higher alcohol biosynthesis by native microorganisms mainly needs gene expression of heterologous keto acid decarboxylase and alcohol dehydrogenases. In the present study, branched-chain alpha-keto acid decarboxylase gene from Lactococcus lactis subsp. lactis CICC 6246 (Kivd) and alcohol dehydrogenases gene from Zymomonas mobilis CICC 41465 (AdhB) were transformed into Escherichia coli for higher alcohol production. SDS-PAGE results showed these two proteins were expressed in the recombinant strains. The resulting strain was incubated in LB medium at 37 A degrees C in Erlenmeyer flasks and much more 3-methyl-1-butanol (104 mg/L) than isobutanol (24 mg/L) was produced. However, in 5 g/L glucose-containing medium, the production of two alcohols was similar, 156 and 161 mg/L for C4 (isobutanol) and C5 (3-methyl-1-butanol) alcohol, respectively. Effects of fermentation factors including temperature, glucose content, and alpha-keto acid on alcohol production were also investigated. The increase of glucose content and the adding of alpha-keto acids facilitated the production of C4 and C5 alcohols. The enzyme activities of pure Kivd on alpha-ketoisovalerate and alpha-ketoisocaproate were 26.77 and 21.24 mu mol min(-1) mg(-1), respectively. Due to its ability on decarboxylation of alpha-ketoisovalerate and alpha-ketoisocaproate, the recombinant E. coli strain showed potential application on isoamyl alcohol and isobutanol production.
文章类型Article
关键词3-methyl-1-butanol Isobutanol Alpha-keto Acid Decarboxylase Alcohol Dehydrogenase
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10295-015-1656-z
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]KETO ACID DECARBOXYLASE ; SACCHAROMYCES-CEREVISIAE ; ISOBUTANOL PRODUCTION ; LACTOCOCCUS-LACTIS ; PHENYLPYRUVATE DECARBOXYLASE ; CORYNEBACTERIUM-GLUTAMICUM ; BIOSYNTHESIS ; METABOLISM ; ENZYME ; IDENTIFICATION
收录类别SCI
语种英语
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000362964900005
引用统计
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/10873
专题中国科学院广州能源研究所
作者单位Chinese Acad Sci, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China
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GB/T 7714
Chen, Xiaoyan,Xu, Jingliang,Yang, Liu,et al. Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(11):1473-1479.
APA Chen, Xiaoyan.,Xu, Jingliang.,Yang, Liu.,Yuan, Zhenhong.,Xiao, Shiyuan.,...&Guo, Ying.(2015).Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(11),1473-1479.
MLA Chen, Xiaoyan,et al."Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.11(2015):1473-1479.
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