Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell | |
Yong, Xiao-Yu1,2; Feng, Jiao1; Chen, Yi-Lu1; Shi, Dong-Yan1; Xu, Yu-Shang1; Zhou, Jun1,2; Wang, Shu-Ya1,2; Xu, Lin1; Yong, Yang-Chun3; Sun, Yong-Ming4; Shi, Chen-Lu5; OuYang, Ping-Kai1,2; Zheng, Tao1,2 | |
2014-06-15 | |
发表期刊 | BIOSENSORS & BIOELECTRONICS |
卷号 | 56页码:19-25 |
摘要 | Microbial fuel cells (MFCs) are promising for harnessing bioenergy from various organic wastes. However, low electricity power output (EPT) is one of the major bottlenecks in the practical application of MFCs. In this study, EPT improvement by cofactor manipulation was explored in the Pseudomonas aeruginosainoculated MFCs. By overexpression of nadE (NAD synthetase gene), the availability of the intracellular cofactor pool (NAD(H/(+))) significantly increased, and delivered approximately three times higher power output than the original strain (increased from 10.86 mu W/cm(2) to 40.13 mu W/cm(2)). The nadE overexpression strain showed about a onefold decrease in charge transfer resistance and higher electrochemical activity than the original strain, which should underlie the power output improvement. Furthermore, cyclic voltammetry, HPLC, and LC-MS analysis showed that the concentration of the electron shuttle (pyocyanin) increased approximately 1.5 fold upon nadE overexpression, which was responsible for the enhanced electrochemical activity. Thus, the results substantiated that the manipulation of intracellular cofactor could be an efficient approach to improve the EPT of MFCs, and implied metabolic engineering is of great potential for EPT improvement. (C) 2013 Elsevier B.V. All rights reserved. |
文章类型 | Article |
关键词 | Microbial Fuel Cell Pseudomonas Aeruginosa Nad Synthetase Nadh/nad(+) Metabolic Engineering |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1016/j.bios.2013.12.058 |
研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
关键词[WOS] | METABOLIC FLUX REDISTRIBUTION ; INCREASING NADH AVAILABILITY ; ESCHERICHIA-COLI ; WASTE-WATER ; SACCHAROMYCES-CEREVISIAE ; HETEROLOGOUS EXPRESSION ; ELECTRICITY-GENERATION ; NADH/NAD(+) RATIO ; ELECTRON-TRANSFER ; POWER PRODUCTION |
收录类别 | SCI |
语种 | 英语 |
WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
WOS记录号 | WOS:000333781000004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.giec.ac.cn/handle/344007/10786 |
专题 | 中国科学院广州能源研究所 |
作者单位 | 1.Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Jiangsu, Peoples R China 2.Nanjing Univ Technol, Bioenergy Res Inst, Nanjing 210009, Jiangsu, Peoples R China 3.Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang 212013, Peoples R China 4.Chinese Acad Sci, Key Laboratoly Renewable Energy, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 5.Guangdong Inst Ecoenvironm & Soli Sci, Guangzhou 510650, Guangdong, Peoples R China |
推荐引用方式 GB/T 7714 | Yong, Xiao-Yu,Feng, Jiao,Chen, Yi-Lu,et al. Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell[J]. BIOSENSORS & BIOELECTRONICS,2014,56:19-25. |
APA | Yong, Xiao-Yu.,Feng, Jiao.,Chen, Yi-Lu.,Shi, Dong-Yan.,Xu, Yu-Shang.,...&Zheng, Tao.(2014).Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell.BIOSENSORS & BIOELECTRONICS,56,19-25. |
MLA | Yong, Xiao-Yu,et al."Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell".BIOSENSORS & BIOELECTRONICS 56(2014):19-25. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论