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Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell
Yong, Xiao-Yu1,2; Feng, Jiao1; Chen, Yi-Lu1; Shi, Dong-Yan1; Xu, Yu-Shang1; Zhou, Jun1,2; Wang, Shu-Ya1,2; Xu, Lin1; Yong, Yang-Chun3; Sun, Yong-Ming4; Shi, Chen-Lu5; OuYang, Ping-Kai1,2; Zheng, Tao1,2
2014-06-15
发表期刊BIOSENSORS & BIOELECTRONICS
卷号56页码:19-25
摘要Microbial fuel cells (MFCs) are promising for harnessing bioenergy from various organic wastes. However, low electricity power output (EPT) is one of the major bottlenecks in the practical application of MFCs. In this study, EPT improvement by cofactor manipulation was explored in the Pseudomonas aeruginosainoculated MFCs. By overexpression of nadE (NAD synthetase gene), the availability of the intracellular cofactor pool (NAD(H/(+))) significantly increased, and delivered approximately three times higher power output than the original strain (increased from 10.86 mu W/cm(2) to 40.13 mu W/cm(2)). The nadE overexpression strain showed about a onefold decrease in charge transfer resistance and higher electrochemical activity than the original strain, which should underlie the power output improvement. Furthermore, cyclic voltammetry, HPLC, and LC-MS analysis showed that the concentration of the electron shuttle (pyocyanin) increased approximately 1.5 fold upon nadE overexpression, which was responsible for the enhanced electrochemical activity. Thus, the results substantiated that the manipulation of intracellular cofactor could be an efficient approach to improve the EPT of MFCs, and implied metabolic engineering is of great potential for EPT improvement. (C) 2013 Elsevier B.V. All rights reserved.
文章类型Article
关键词Microbial Fuel Cell Pseudomonas Aeruginosa Nad Synthetase Nadh/nad(+) Metabolic Engineering
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.bios.2013.12.058
研究领域[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]METABOLIC FLUX REDISTRIBUTION ; INCREASING NADH AVAILABILITY ; ESCHERICHIA-COLI ; WASTE-WATER ; SACCHAROMYCES-CEREVISIAE ; HETEROLOGOUS EXPRESSION ; ELECTRICITY-GENERATION ; NADH/NAD(+) RATIO ; ELECTRON-TRANSFER ; POWER PRODUCTION
收录类别SCI
语种英语
WOS类目Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号WOS:000333781000004
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/10786
专题中国科学院广州能源研究所
作者单位1.Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Jiangsu, Peoples R China
2.Nanjing Univ Technol, Bioenergy Res Inst, Nanjing 210009, Jiangsu, Peoples R China
3.Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang 212013, Peoples R China
4.Chinese Acad Sci, Key Laboratoly Renewable Energy, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
5.Guangdong Inst Ecoenvironm & Soli Sci, Guangzhou 510650, Guangdong, Peoples R China
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GB/T 7714
Yong, Xiao-Yu,Feng, Jiao,Chen, Yi-Lu,et al. Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell[J]. BIOSENSORS & BIOELECTRONICS,2014,56:19-25.
APA Yong, Xiao-Yu.,Feng, Jiao.,Chen, Yi-Lu.,Shi, Dong-Yan.,Xu, Yu-Shang.,...&Zheng, Tao.(2014).Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell.BIOSENSORS & BIOELECTRONICS,56,19-25.
MLA Yong, Xiao-Yu,et al."Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell".BIOSENSORS & BIOELECTRONICS 56(2014):19-25.
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