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Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell
Yong, Xiao-Yu1,2; Feng, Jiao1; Chen, Yi-Lu1; Shi, Dong-Yan1; Xu, Yu-Shang1; Zhou, Jun1,2; Wang, Shu-Ya1,2; Xu, Lin1; Yong, Yang-Chun3; Sun, Yong-Ming4; Shi, Chen-Lu5; OuYang, Ping-Kai1,2; Zheng, Tao1,2
2014-06-15
Source PublicationBIOSENSORS & BIOELECTRONICS
Volume56Pages:19-25
AbstractMicrobial fuel cells (MFCs) are promising for harnessing bioenergy from various organic wastes. However, low electricity power output (EPT) is one of the major bottlenecks in the practical application of MFCs. In this study, EPT improvement by cofactor manipulation was explored in the Pseudomonas aeruginosainoculated MFCs. By overexpression of nadE (NAD synthetase gene), the availability of the intracellular cofactor pool (NAD(H/(+))) significantly increased, and delivered approximately three times higher power output than the original strain (increased from 10.86 mu W/cm(2) to 40.13 mu W/cm(2)). The nadE overexpression strain showed about a onefold decrease in charge transfer resistance and higher electrochemical activity than the original strain, which should underlie the power output improvement. Furthermore, cyclic voltammetry, HPLC, and LC-MS analysis showed that the concentration of the electron shuttle (pyocyanin) increased approximately 1.5 fold upon nadE overexpression, which was responsible for the enhanced electrochemical activity. Thus, the results substantiated that the manipulation of intracellular cofactor could be an efficient approach to improve the EPT of MFCs, and implied metabolic engineering is of great potential for EPT improvement. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicrobial Fuel Cell Pseudomonas Aeruginosa Nad Synthetase Nadh/nad(+) Metabolic Engineering
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.bios.2013.12.058
WOS Subject ExtendedBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS KeywordMETABOLIC FLUX REDISTRIBUTION ; INCREASING NADH AVAILABILITY ; ESCHERICHIA-COLI ; WASTE-WATER ; SACCHAROMYCES-CEREVISIAE ; HETEROLOGOUS EXPRESSION ; ELECTRICITY-GENERATION ; NADH/NAD(+) RATIO ; ELECTRON-TRANSFER ; POWER PRODUCTION
Indexed BySCI
Language英语
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000333781000004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10786
Collection中国科学院广州能源研究所
Affiliation1.Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Jiangsu, Peoples R China
2.Nanjing Univ Technol, Bioenergy Res Inst, Nanjing 210009, Jiangsu, Peoples R China
3.Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang 212013, Peoples R China
4.Chinese Acad Sci, Key Laboratoly Renewable Energy, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
5.Guangdong Inst Ecoenvironm & Soli Sci, Guangzhou 510650, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Yong, Xiao-Yu,Feng, Jiao,Chen, Yi-Lu,et al. Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell[J]. BIOSENSORS & BIOELECTRONICS,2014,56:19-25.
APA Yong, Xiao-Yu.,Feng, Jiao.,Chen, Yi-Lu.,Shi, Dong-Yan.,Xu, Yu-Shang.,...&Zheng, Tao.(2014).Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell.BIOSENSORS & BIOELECTRONICS,56,19-25.
MLA Yong, Xiao-Yu,et al."Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell".BIOSENSORS & BIOELECTRONICS 56(2014):19-25.
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