Kinetics and Mechanism Analysis on Microbial Oil Production by Trichosporon fermentans in Rice Straw Hydrolysate

doi:10.1021/ie502185u

GIEC OpenIR > 中国科学院广州能源研究所

	Kinetics and Mechanism Analysis on Microbial Oil Production by Trichosporon fermentans in Rice Straw Hydrolysate
	Huang, Chao 1,2,3; Li, Yuan-yuan 1; Liu, Li-ping 1; Wu, Hong 1; Zong, Min-hua 1; Lou, Wen-yong 1
	2014-12-10
发表期刊	INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
卷号	53 期号:49 页码:19034-19043
摘要	To give an insight into the kinetics of microbial oil fermentation with lignocellulosic hydrolysate as substrate, lipid production by Trichosporon fermentans in rice straw hydrolysate was investigated in a 5 L fermentor. For comparison, fermentation in its simulated medium was also performed to evaluate the effect of inhibitors present in the rice straw hydrolysate on the cell growth and lipid accumulation of T. fermentans. The optimum fermentation time, maximum biomass, and lipid content of T. fermentans in the rice straw hydrolysate were 10.5 days, 28.6 g/L, and 43.9%, respectively, while the corresponding values in the simulated medium were 8.5 days, 27.0 g/L, and 65.0%, respectively, indicating that the inhibitors in the rice straw hydrolysate did show some inhibition on the cell growth and lipid accumulation of T. fermentans. Kinetic models of lipid fermentation with T. fermentans showed the maximum specific growth rate and the correlation coefficient of lipid synthesis to cell growth in the rice straw hydrolysate were 0.40 and 0.51, respectively, which are much lower than those in the simulated medium (0.58 and 0.73). To better understand the influential mechanism of inhibitors in the rice straw hydrolysate on the growth and lipid accumulation of T. fermentans, the physiological and biochemical changes of cells in the fermentation were further investigated. The reduced activities of adenosine triphosphate (ATP) citrate lyase, malic enzyme, and xylose reductase, and the elongation of cells at the beginning of fermentation, partly account for the inhibitory effect of inhibitors in the rice straw hydrolysate.
文章类型	Article
WOS标题词	Science & Technology ; Technology
DOI	10.1021/ie502185u
研究领域[WOS]	Engineering
关键词[WOS]	LIPID-ACCUMULATION ; SACCHAROMYCES-CEREVISIAE ; YEAST ; GROWTH ; BIOFUELS ; XYLOSE
收录类别	SCI
语种	英语
WOS类目	Engineering, Chemical
WOS记录号	WOS:000346322200046
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/10624
专题	中国科学院广州能源研究所
作者单位	1.S China Univ Technol, Coll Light Ind & Food Sci, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Huang, Chao,Li, Yuan-yuan,Liu, Li-ping,et al. Kinetics and Mechanism Analysis on Microbial Oil Production by Trichosporon fermentans in Rice Straw Hydrolysate[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2014,53(49):19034-19043.
APA	Huang, Chao,Li, Yuan-yuan,Liu, Li-ping,Wu, Hong,Zong, Min-hua,&Lou, Wen-yong.(2014).Kinetics and Mechanism Analysis on Microbial Oil Production by Trichosporon fermentans in Rice Straw Hydrolysate.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,53(49),19034-19043.
MLA	Huang, Chao,et al."Kinetics and Mechanism Analysis on Microbial Oil Production by Trichosporon fermentans in Rice Straw Hydrolysate".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 53.49(2014):19034-19043.