L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum

doi:10.1007/s00726-012-1308-9

GIEC OpenIR > 中国科学院广州能源研究所

	L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum
	Hou, Xiaohu 1,2,3; Chen, Xinde1,2 ; Zhang, Yue 3; Qian, He 3; Zhang, Weiguo 3
	2012-12-01
发表期刊	AMINO ACIDS
卷号	43 期号:6 页码:2301-2311
摘要	Corynebacterium glutamicum ATCC13032 and Brevibacterium flavum JV16 were engineered for L-valine production by over-expressing ilvEBN(r)C genes at 31 degrees C in 72 h fermentation. Different strategies were carried out to reduce the by-products' accumulation in L-valine fermentation and also to increase the availability of precursor for L-valine biosynthesis. The native promoter of ilvA of C. glutamicum was replaced with a weak promoter MPilvA (P-ilvAM1CG) to reduce the biosynthetic rate of L-isoleucine. Effect of different relative dissolved oxygen on L-valine production and by-products' formation was recorded, indicating that 15 % saturation may be the most appropriate relative dissolved oxygen for L-valine fermentation with almost no L-lactic acid and L-glutamate formed. To minimize L-alanine accumulation, alaT and/or avtA was inactivated in C. glutamicum and B. flavum, respectively. Compared to high concentration of L-alanine accumulated by alaT inactivated strains harboring ilvEBN(r)C genes, L-alanine concentration was reduced to 0.18 g/L by C. glutamicum ATCC13032MPilvA(Delta)avtA pDXW-8-ilvEBN(r)C, and 0.22 g/L by B. flavum JV16avtA::Cm pDXW-8-ilvEBN(r)C. Meanwhile, L-valine production and conversion efficiency were enhanced to 31.15 g/L and 0.173 g/g by C. glutamicum ATCC13032MPilvA(Delta)avtA pDXW-8-ilvEBN(r)C, 38.82 g/L and 0.252 g/g by B. flavum JV16avtA::Cm pDXW-8-ilvEBN(r)C. This study provides combined strategies to improve L-valine yield by minimization of by-products' production.
文章类型	Article
关键词	L-valine Corynebacterium Glutamicum Brevibacterium Flavum Alat Avta
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00726-012-1308-9
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	ESCHERICHIA-COLI ; AMINO-ACIDS ; METABOLISM ; PATHWAY ; GENES
收录类别	SCI
语种	英语
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000310885500008
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/10563
专题	中国科学院广州能源研究所集成技术研发中心
作者单位	1.Chinese Acad Sci, Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 3.JiangNan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Hou, Xiaohu,Chen, Xinde,Zhang, Yue,et al. L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum[J]. AMINO ACIDS,2012,43(6):2301-2311.
APA	Hou, Xiaohu,Chen, Xinde,Zhang, Yue,Qian, He,&Zhang, Weiguo.(2012).L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum.AMINO ACIDS,43(6),2301-2311.
MLA	Hou, Xiaohu,et al."L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum".AMINO ACIDS 43.6(2012):2301-2311.