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L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum
Hou, Xiaohu1,2,3; Chen, Xinde1,2; Zhang, Yue3; Qian, He3; Zhang, Weiguo3
2012-12-01
Source PublicationAMINO ACIDS
Volume43Issue:6Pages:2301-2311
AbstractCorynebacterium glutamicum ATCC13032 and Brevibacterium flavum JV16 were engineered for L-valine production by over-expressing ilvEBN(r)C genes at 31 degrees C in 72 h fermentation. Different strategies were carried out to reduce the by-products' accumulation in L-valine fermentation and also to increase the availability of precursor for L-valine biosynthesis. The native promoter of ilvA of C. glutamicum was replaced with a weak promoter MPilvA (P-ilvAM1CG) to reduce the biosynthetic rate of L-isoleucine. Effect of different relative dissolved oxygen on L-valine production and by-products' formation was recorded, indicating that 15 % saturation may be the most appropriate relative dissolved oxygen for L-valine fermentation with almost no L-lactic acid and L-glutamate formed. To minimize L-alanine accumulation, alaT and/or avtA was inactivated in C. glutamicum and B. flavum, respectively. Compared to high concentration of L-alanine accumulated by alaT inactivated strains harboring ilvEBN(r)C genes, L-alanine concentration was reduced to 0.18 g/L by C. glutamicum ATCC13032MPilvA(Delta)avtA pDXW-8-ilvEBN(r)C, and 0.22 g/L by B. flavum JV16avtA::Cm pDXW-8-ilvEBN(r)C. Meanwhile, L-valine production and conversion efficiency were enhanced to 31.15 g/L and 0.173 g/g by C. glutamicum ATCC13032MPilvA(Delta)avtA pDXW-8-ilvEBN(r)C, 38.82 g/L and 0.252 g/g by B. flavum JV16avtA::Cm pDXW-8-ilvEBN(r)C. This study provides combined strategies to improve L-valine yield by minimization of by-products' production.
SubtypeArticle
KeywordL-valine Corynebacterium Glutamicum Brevibacterium Flavum Alat Avta
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00726-012-1308-9
WOS Subject ExtendedBiochemistry & Molecular Biology
WOS KeywordESCHERICHIA-COLI ; AMINO-ACIDS ; METABOLISM ; PATHWAY ; GENES
Indexed BySCI
Language英语
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000310885500008
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10563
Collection中国科学院广州能源研究所
集成技术研发中心
Affiliation1.Chinese Acad Sci, Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
3.JiangNan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Hou, Xiaohu,Chen, Xinde,Zhang, Yue,et al. L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum[J]. AMINO ACIDS,2012,43(6):2301-2311.
APA Hou, Xiaohu,Chen, Xinde,Zhang, Yue,Qian, He,&Zhang, Weiguo.(2012).L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum.AMINO ACIDS,43(6),2301-2311.
MLA Hou, Xiaohu,et al."L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum".AMINO ACIDS 43.6(2012):2301-2311.
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