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Characterization of direct cellulase immobilization with superparamagnetic nanoparticles
Xu, Jingliang1; Huo, Shuhao2; Yuan, Zhenhong1; Zhang, Yu1; Xu, Huijuan1; Guo, Ying1; Liang, Cuiyi1; Zhuang, Xinshu1
2011-03-01
Source PublicationBIOCATALYSIS AND BIOTRANSFORMATION
Volume29Issue:2-3Pages:71-76
AbstractMethods of cellulase immobilization on magnetic particles via glutaraldehyde binding were studied. The binding was confirmed by transmission electronic microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and vibrating sample magnetometry (VSM). Samples analyzed by TEM and XRD showed that the magnetic particles with or without bound cellulase were all nanosized particles with a mean diameter of 11.5 nm, and the binding process did not cause significant changes in particle size and structure. Analysis by FTIR showed that the binding of cellulase to the magnetic nanoparticles might be via covalent bonding between residual amine groups on Fe(3)O(4) nanoparticles and amine groups of the cellulase. The VSM analysis showed that magnetic nanoparticles with or without bound cellulase were all superparamagnetic. The immobilized cellulase had a wider pH and temperature range and improved storage stability compared with the free enzyme. Determination of the Michaelis constants revealed that the immobilized cellulase had a greater affinity for the cellulosic substrate than the free enzyme. The immobilized cellulase showed better performance on hydrolysis of steam-exploded corn stalks than of bleached sulfite bagasse pulp.
SubtypeArticle
KeywordCellulase Direct Immobilization Superparamagnetic Nanoparticles Glutaraldehyde
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.3109/10242422.2011.566326
WOS Subject ExtendedBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS KeywordDIRECT BINDING PROCEDURE ; MAGNETIC NANOPARTICLES ; PARTICLES ; DEHYDROGENASE ; STABILITY
Indexed BySCI
Language英语
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000290868200004
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10413
Collection中国科学院广州能源研究所
Affiliation1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China
2.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Xu, Jingliang,Huo, Shuhao,Yuan, Zhenhong,et al. Characterization of direct cellulase immobilization with superparamagnetic nanoparticles[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2011,29(2-3):71-76.
APA Xu, Jingliang.,Huo, Shuhao.,Yuan, Zhenhong.,Zhang, Yu.,Xu, Huijuan.,...&Zhuang, Xinshu.(2011).Characterization of direct cellulase immobilization with superparamagnetic nanoparticles.BIOCATALYSIS AND BIOTRANSFORMATION,29(2-3),71-76.
MLA Xu, Jingliang,et al."Characterization of direct cellulase immobilization with superparamagnetic nanoparticles".BIOCATALYSIS AND BIOTRANSFORMATION 29.2-3(2011):71-76.
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