Characterization of direct cellulase immobilization with superparamagnetic nanoparticles

doi:10.3109/10242422.2011.566326

GIEC OpenIR > 中国科学院广州能源研究所

	Characterization of direct cellulase immobilization with superparamagnetic nanoparticles
	Xu, Jingliang 1; Huo, Shuhao 2; Yuan, Zhenhong 1; Zhang, Yu 1; Xu, Huijuan 1; Guo, Ying 1; Liang, Cuiyi 1; Zhuang, Xinshu 1
	2011-03-01
发表期刊	BIOCATALYSIS AND BIOTRANSFORMATION
卷号	29 期号:2-3 页码:71-76
摘要	Methods of cellulase immobilization on magnetic particles via glutaraldehyde binding were studied. The binding was confirmed by transmission electronic microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and vibrating sample magnetometry (VSM). Samples analyzed by TEM and XRD showed that the magnetic particles with or without bound cellulase were all nanosized particles with a mean diameter of 11.5 nm, and the binding process did not cause significant changes in particle size and structure. Analysis by FTIR showed that the binding of cellulase to the magnetic nanoparticles might be via covalent bonding between residual amine groups on Fe(3)O(4) nanoparticles and amine groups of the cellulase. The VSM analysis showed that magnetic nanoparticles with or without bound cellulase were all superparamagnetic. The immobilized cellulase had a wider pH and temperature range and improved storage stability compared with the free enzyme. Determination of the Michaelis constants revealed that the immobilized cellulase had a greater affinity for the cellulosic substrate than the free enzyme. The immobilized cellulase showed better performance on hydrolysis of steam-exploded corn stalks than of bleached sulfite bagasse pulp.
文章类型	Article
关键词	Cellulase Direct Immobilization Superparamagnetic Nanoparticles Glutaraldehyde
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3109/10242422.2011.566326
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	DIRECT BINDING PROCEDURE ; MAGNETIC NANOPARTICLES ; PARTICLES ; DEHYDROGENASE ; STABILITY
收录类别	SCI
语种	英语
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000290868200004
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/10413
专题	中国科学院广州能源研究所
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China 2.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xu, Jingliang,Huo, Shuhao,Yuan, Zhenhong,et al. Characterization of direct cellulase immobilization with superparamagnetic nanoparticles[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2011,29(2-3):71-76.
APA	Xu, Jingliang.,Huo, Shuhao.,Yuan, Zhenhong.,Zhang, Yu.,Xu, Huijuan.,...&Zhuang, Xinshu.(2011).Characterization of direct cellulase immobilization with superparamagnetic nanoparticles.BIOCATALYSIS AND BIOTRANSFORMATION,29(2-3),71-76.
MLA	Xu, Jingliang,et al."Characterization of direct cellulase immobilization with superparamagnetic nanoparticles".BIOCATALYSIS AND BIOTRANSFORMATION 29.2-3(2011):71-76.