Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary | |
| Zhang Chunsun; Xu Jinliang; Wang Jianqin; Wang Hanping | |
| 2006-08-01 | |
| Source Publication | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| Volume | 34Issue:8Pages:1197-1202 |
| Abstract | The polytetrafluoroethylene ( PTFE ) capillary-based continuous-flow polymerase chain reaction ( PCR ) microfluidics was constructed by using the flexible thin film heaters to form three constant temperature zones necessary for a PCR amplification, in which the PCR mixture could flow through the capillary microchannel continuously, thus leading,to the successful completion of PCR exponential amplification. The effects of various concentrations of bovine serum albumin ( BSA ) in a 25 mu L PCR mixture and flow rates of PCR mixture in the capillary microchannel, on continuous-flow PCR amplification, had been discussed in detail, where a PCR amplification of 249 base pair (bp) human beta-actin gene fragments was successfully performed within 15 and even 10 minutes, which is several times faster than the reaction time required ( 1 - 2 h) with the conventional PCR machine. |
| Subtype | Article |
| Keyword | Capillary Continuous-flow Polymerase Chain Reaction Microfluidics |
| WOS Headings | Science & Technology ; Physical Sciences |
| WOS Subject Extended | Chemistry |
| WOS Keyword | DNA AMPLIFICATION ; PCR CHIP ; DEVICE ; POLYCARBONATE |
| Indexed By | SCI |
| Language | 英语 |
| WOS Subject | Chemistry, Analytical |
| WOS ID | WOS:000243165100036 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/10263 |
| Collection | 中国科学院广州能源研究所 |
| Affiliation | 1.Chinese Acad Sci, Micro Energy Syst Lab, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 2.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Anhua 230026, Peoples R China 3.Guangzhou First Municipal Peoples Hosp, Guangzhou 510180, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang Chunsun,Xu Jinliang,Wang Jianqin,et al. Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(8):1197-1202. |
| APA | Zhang Chunsun,Xu Jinliang,Wang Jianqin,&Wang Hanping.(2006).Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(8),1197-1202. |
| MLA | Zhang Chunsun,et al."Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.8(2006):1197-1202. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment