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Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary
Zhang Chunsun; Xu Jinliang; Wang Jianqin; Wang Hanping
2006-08-01
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号34期号:8页码:1197-1202
摘要The polytetrafluoroethylene ( PTFE ) capillary-based continuous-flow polymerase chain reaction ( PCR ) microfluidics was constructed by using the flexible thin film heaters to form three constant temperature zones necessary for a PCR amplification, in which the PCR mixture could flow through the capillary microchannel continuously, thus leading,to the successful completion of PCR exponential amplification. The effects of various concentrations of bovine serum albumin ( BSA ) in a 25 mu L PCR mixture and flow rates of PCR mixture in the capillary microchannel, on continuous-flow PCR amplification, had been discussed in detail, where a PCR amplification of 249 base pair (bp) human beta-actin gene fragments was successfully performed within 15 and even 10 minutes, which is several times faster than the reaction time required ( 1 - 2 h) with the conventional PCR machine.
文章类型Article
关键词Capillary Continuous-flow Polymerase Chain Reaction Microfluidics
WOS标题词Science & Technology ; Physical Sciences
研究领域[WOS]Chemistry
关键词[WOS]DNA AMPLIFICATION ; PCR CHIP ; DEVICE ; POLYCARBONATE
收录类别SCI
语种英语
WOS类目Chemistry, Analytical
WOS记录号WOS:000243165100036
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/10263
专题中国科学院广州能源研究所
作者单位1.Chinese Acad Sci, Micro Energy Syst Lab, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China
2.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Anhua 230026, Peoples R China
3.Guangzhou First Municipal Peoples Hosp, Guangzhou 510180, Peoples R China
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Zhang Chunsun,Xu Jinliang,Wang Jianqin,et al. Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(8):1197-1202.
APA Zhang Chunsun,Xu Jinliang,Wang Jianqin,&Wang Hanping.(2006).Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(8),1197-1202.
MLA Zhang Chunsun,et al."Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.8(2006):1197-1202.
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