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Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary
Zhang Chunsun; Xu Jinliang; Wang Jianqin; Wang Hanping
2006-08-01
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
Volume34Issue:8Pages:1197-1202
AbstractThe polytetrafluoroethylene ( PTFE ) capillary-based continuous-flow polymerase chain reaction ( PCR ) microfluidics was constructed by using the flexible thin film heaters to form three constant temperature zones necessary for a PCR amplification, in which the PCR mixture could flow through the capillary microchannel continuously, thus leading,to the successful completion of PCR exponential amplification. The effects of various concentrations of bovine serum albumin ( BSA ) in a 25 mu L PCR mixture and flow rates of PCR mixture in the capillary microchannel, on continuous-flow PCR amplification, had been discussed in detail, where a PCR amplification of 249 base pair (bp) human beta-actin gene fragments was successfully performed within 15 and even 10 minutes, which is several times faster than the reaction time required ( 1 - 2 h) with the conventional PCR machine.
SubtypeArticle
KeywordCapillary Continuous-flow Polymerase Chain Reaction Microfluidics
WOS HeadingsScience & Technology ; Physical Sciences
WOS Subject ExtendedChemistry
WOS KeywordDNA AMPLIFICATION ; PCR CHIP ; DEVICE ; POLYCARBONATE
Indexed BySCI
Language英语
WOS SubjectChemistry, Analytical
WOS IDWOS:000243165100036
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10263
Collection中国科学院广州能源研究所
Affiliation1.Chinese Acad Sci, Micro Energy Syst Lab, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China
2.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Anhua 230026, Peoples R China
3.Guangzhou First Municipal Peoples Hosp, Guangzhou 510180, Peoples R China
Recommended Citation
GB/T 7714
Zhang Chunsun,Xu Jinliang,Wang Jianqin,et al. Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(8):1197-1202.
APA Zhang Chunsun,Xu Jinliang,Wang Jianqin,&Wang Hanping.(2006).Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(8),1197-1202.
MLA Zhang Chunsun,et al."Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.8(2006):1197-1202.
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