The alleviation of lignin inhibition on enzymatic hydrolysis of cellulose by its ultrastructure

doi:10.1016/j.indcrop.2022.115108

GIEC OpenIR

	The alleviation of lignin inhibition on enzymatic hydrolysis of cellulose by its ultrastructure
	Chen, Xindong 1,5; Li, Hailong 1,2,3,4; Yao, Shimiao 1,2,3,4; Wang, Can 1,2,3,4; Chen, Xuefang 1,2,3,4; Guo, Haijun 1,2,3,4; Xiong, Lian1,2,3,4 ; Zhang, Hairong 1,2,3,4; Chen, Xinde1,2,3,4
	2022-10-01
发表期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
卷号	185 页码:8
通讯作者	Li, Hailong(lihl@ms.giec.ac.cn) ; Chen, Xinde(cxd_cxd@hotmail.com)
摘要	Lignin is regarded as the primary factor that inhibits enzymatic hydrolysis of cellulose. However, the impact of cellulose ultrastructure on the inhibition of lignin during enzymatic hydrolysis is still unclear. In this work, the addition of lignin during enzymatic hydrolysis of cellulose with different ultrastructure was investigated. Furthermore, the influences of changing cellulose ultrastructure on cellulose conversion of pine after hydro thermal treatment (HT) without and with additives (2-naphthol (2 N) and phloroglucinol (PL)) were explored in detail. The results showed that enzymatic hydrolyzability of allomorphic cellulose was strongly inhibited by lignin. In contrast, a small inhibition degree of lignin was observed for amorphous cellulose. Surprisingly, the cellulose conversion of HT treated pine was dramatically decreased (18.45-6.25%) after ball-milling treatment (BM), while it was significantly increased (18.45-74.49%) after concentrated phosphoric acid treatment (CP). In addition, the addition of 2 N or PL during HT could promote or inhibit cellulose conversion due to the changes in the condensation degree of lignin. These results indicated that the physiochemical structure of lignin has a significant effect on cellulose hydrolysis. More importantly, the increasing degree of cellulose conversion was enhanced in sequence from HT+PL (6.19-35.00%), HT (18.45-74.49%), to HT+ 2 N (21.12-92.30%) after CP, which was attributed to the reducing lignin condensation and changing cellulose ultrastructure. Overall, this work demonstrated that the inhibition degree of lignin was highly dependent on the cellulose ultrastructure.
关键词	Cellulose ultrastructure Lignin inhibition Enzymatic hydrolysis Cellulase
DOI	10.1016/j.indcrop.2022.115108
关键词[WOS]	AUTOHYDROLYSIS PRETREATMENT ; PSEUDO-LIGNIN ; BIOMASS ; RECALCITRANCE ; CHEMISTRY ; SOFTWOOD
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31600475] ; National Natural Science Foundation of China[51876207] ; Guangdong Basic and Applied Basic Research Foundation[2019A1515011843] ; Project of Jiangsu Province Science and Technology[BE2020394]
WOS研究方向	Agriculture
项目资助者	National Natural Science Foundation of China ; Guangdong Basic and Applied Basic Research Foundation ; Project of Jiangsu Province Science and Technology
WOS类目	Agricultural Engineering ; Agronomy
WOS记录号	WOS:000808530800010
出版者	ELSEVIER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/36884
专题	中国科学院广州能源研究所
通讯作者	Li, Hailong; Chen, Xinde
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 4.R&D Ctr Xuyi Attapulgite Energy& Environm Mat, Lanzhou 211700, Xuyi, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Chen, Xindong,Li, Hailong,Yao, Shimiao,et al. The alleviation of lignin inhibition on enzymatic hydrolysis of cellulose by its ultrastructure[J]. INDUSTRIAL CROPS AND PRODUCTS,2022,185:8.
APA	Chen, Xindong.,Li, Hailong.,Yao, Shimiao.,Wang, Can.,Chen, Xuefang.,...&Chen, Xinde.(2022).The alleviation of lignin inhibition on enzymatic hydrolysis of cellulose by its ultrastructure.INDUSTRIAL CROPS AND PRODUCTS,185,8.
MLA	Chen, Xindong,et al."The alleviation of lignin inhibition on enzymatic hydrolysis of cellulose by its ultrastructure".INDUSTRIAL CROPS AND PRODUCTS 185(2022):8.