Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae

doi:10.1016/j.bej.2021.108198

GIEC OpenIR

	Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae
	Zhang, Yi 1,2,3,4; Kang, Xihui 5; Zhen, Feng1,2,3 ; Wang, Zhongming1,2 ; Kong, Xiaoying1,3 ; Sun, Yongming1,2,3
	2022
发表期刊	BIOCHEMICAL ENGINEERING JOURNAL
ISSN	1369-703X
卷号	177 页码:12
通讯作者	Wang, Zhongming(wangzm@ms.giec.ac.cn) ; Sun, Yongming(sunym@ms.giec.ac.cn)
摘要	Enzymatic hydrolysis is emerging as a promising unconventional pretreatment method for disrupting microalgae cells. However, the sustainability of biological enzyme needs to be improved due to low efficiency of enzymatic hydrolysis with single enzyme. Cellulase, xylanase, pectinase, snailase, lysozyme and trypsin were selected to pretreat microalgae Scenedesmus sp. for lipid extraction. The results shown that the pretreatment of a multi-enzyme combination of cellulase, xylanase and pectinase perform a better enzymatic hydrolysis effect than each single enzyme. Results shown that different sequences of enzymatic hydrolysis had a significant effect on the lipid extraction performance from microalgae, the lipid recovery yield of 100% (total lipids content) could be achieved with the sequence of stepwise enzymatic hydrolysis: pectinase (10 mg g(-1) dry weight microalgae), cellulase (20 mg g(-1)) and xylanase (14 mg g(-1)). The SEM image, FTIR measurement, HPLC and XPS analysis indicated that the outer layer of trilaminar sheaths in the microaglal cell walls was first degraded by pectinase, and then cellulose and hemicellulose in the inner layer were destroyed by cellulase and xylanase. This study provides an efficient and environmental-friendly mute for lipid extraction as a new strategy of enzymatic hydrolysis to valorize high-value added products in microalgae downstream processing.
关键词	Enzymatic hydrolysis Disruption Microalgae cell walls Lipid extraction
DOI	10.1016/j.bej.2021.108198
关键词[WOS]	CELL-WALLS ; AMINO-ACID ; EXTRACTION ; HYDROLYSIS ; OPTIMIZATION ; DISRUPTION ; ALGAE ; PRETREATMENT ; CONVERSION ; PRODUCTS
收录类别	SCI
语种	英语
资助项目	China and Germany Postdoctoral Exchange Program - Office of the China Postdoctoral Council of the Ministry of Human Resources[ZD2018025] ; Natural Science Foundation of Guangdong Province[2017B020238005] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA21050400] ; Guangzhou Science and Technology Project[201803030007] ; China and Germany Postdoctoral Exchange Program - Helmholtz Association[ZD2018025]
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering
项目资助者	China and Germany Postdoctoral Exchange Program - Office of the China Postdoctoral Council of the Ministry of Human Resources ; Natural Science Foundation of Guangdong Province ; Strategic Priority Research Program of Chinese Academy of Sciences ; Guangzhou Science and Technology Project ; China and Germany Postdoctoral Exchange Program - Helmholtz Association
WOS类目	Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS记录号	WOS:000717092700001
出版者	ELSEVIER
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/35268
专题	中国科学院广州能源研究所
通讯作者	Wang, Zhongming; Sun, Yongming
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 4.Karlsruhe Inst Technol, Inst Pulsed Power & Microwave Technol IHM, D-76344 Eggenstein Leopoldshafen, Germany 5.Univ Coll Cork, MaREI Ctr, Environm Res Inst, Cork, Ireland
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Zhang, Yi,Kang, Xihui,Zhen, Feng,et al. Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae[J]. BIOCHEMICAL ENGINEERING JOURNAL,2022,177:12.
APA	Zhang, Yi,Kang, Xihui,Zhen, Feng,Wang, Zhongming,Kong, Xiaoying,&Sun, Yongming.(2022).Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae.BIOCHEMICAL ENGINEERING JOURNAL,177,12.
MLA	Zhang, Yi,et al."Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae".BIOCHEMICAL ENGINEERING JOURNAL 177(2022):12.