Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237

doi:10.15376/biores.15.4.8355-8368

GIEC OpenIR

	Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237
	Liu, Yunyun1,2 ; Xie, Xianli 1; Liu, Wenhuan 1; Xu, Huijuan2 ; Cao, Yunqi 1
	2020-11-01
发表期刊	BIORESOURCES
ISSN	1930-2126
卷号	15 期号:4 页码:8355-8368
通讯作者	Xu, Huijuan(xuhj@ms.giec.ac.cn)
摘要	A consolidated bioprocessing (CBP) using Clostridium thermocellum DSM 1237 for bioethanol production in anaerobic bottles and a 3-L fermenter from biomass was investigated. The effects of key operational parameters including different carbon sources, temperature, and substrates on the metabolic performance of the strain were firstly evaluated. It was found that ethanol yield reached 0.60 g/L with a cell biomass of 0.80 g/g at the optimal temperature of 60 degrees C with 0.5% (w/v) cellobiose. Further experiments indicated that sugarcane bagasse (SCB) could be utilized to efficiently culture this strain. Ethanol yield reached 0.68 g/L (65.8% of theoretical yield) from alkali-pretreated SCB. In the subsequent 3-L fermenter trial, the maximum ethanol 0.86 g/L (83.3% of theoretical yield) was achieved, with enzymes enriched in both cellulase and xylanase. The CBP provided enzymes on-site and integrated hydrolysis and fermentation in one-step, which might be an effective approach for economic bioethanol production.
关键词	Clostridium thermocellum Consolidate bioprocess Bioethanol Sugarcane bagasse Fementer
DOI	10.15376/biores.15.4.8355-8368
关键词[WOS]	CELLULOSIC ETHANOL
收录类别	SCI
语种	英语
资助项目	Program of Science & Technology Planning Project of Xi' an city[20193039YF027NS027] ; Natural Science Foundation of Shaanxi Province[2018JQ5134] ; National Natural Science Foundation of China[51876206] ; National Key Research and Development Program[2019YFB1503904] ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources[SKLCUSA-b201802] ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion[Y907k51001]
WOS研究方向	Materials Science
项目资助者	Program of Science & Technology Planning Project of Xi' an city ; Natural Science Foundation of Shaanxi Province ; National Natural Science Foundation of China ; National Key Research and Development Program ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion
WOS类目	Materials Science, Paper & Wood
WOS记录号	WOS:000583802900074
出版者	NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/34847
专题	中国科学院广州能源研究所
通讯作者	Xu, Huijuan
作者单位	1.Shaanxi Univ Sci & Technol, Coll Mech & Elect Engn, Xian 710021, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Liu, Yunyun,Xie, Xianli,Liu, Wenhuan,et al. Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237[J]. BIORESOURCES,2020,15(4):8355-8368.
APA	Liu, Yunyun,Xie, Xianli,Liu, Wenhuan,Xu, Huijuan,&Cao, Yunqi.(2020).Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237.BIORESOURCES,15(4),8355-8368.
MLA	Liu, Yunyun,et al."Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237".BIORESOURCES 15.4(2020):8355-8368.