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Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237
Liu, Yunyun1,2; Xie, Xianli1; Liu, Wenhuan1; Xu, Huijuan2; Cao, Yunqi1
2020-11-01
Source PublicationBIORESOURCES
ISSN1930-2126
Volume15Issue:4Pages:8355-8368
Corresponding AuthorXu, Huijuan(xuhj@ms.giec.ac.cn)
AbstractA consolidated bioprocessing (CBP) using Clostridium thermocellum DSM 1237 for bioethanol production in anaerobic bottles and a 3-L fermenter from biomass was investigated. The effects of key operational parameters including different carbon sources, temperature, and substrates on the metabolic performance of the strain were firstly evaluated. It was found that ethanol yield reached 0.60 g/L with a cell biomass of 0.80 g/g at the optimal temperature of 60 degrees C with 0.5% (w/v) cellobiose. Further experiments indicated that sugarcane bagasse (SCB) could be utilized to efficiently culture this strain. Ethanol yield reached 0.68 g/L (65.8% of theoretical yield) from alkali-pretreated SCB. In the subsequent 3-L fermenter trial, the maximum ethanol 0.86 g/L (83.3% of theoretical yield) was achieved, with enzymes enriched in both cellulase and xylanase. The CBP provided enzymes on-site and integrated hydrolysis and fermentation in one-step, which might be an effective approach for economic bioethanol production.
KeywordClostridium thermocellum Consolidate bioprocess Bioethanol Sugarcane bagasse Fementer
DOI10.15376/biores.15.4.8355-8368
WOS KeywordCELLULOSIC ETHANOL
Indexed BySCI
Language英语
Funding ProjectProgram of Science & Technology Planning Project of Xi' an city[20193039YF027NS027] ; Natural Science Foundation of Shaanxi Province[2018JQ5134] ; National Natural Science Foundation of China[51876206] ; National Key Research and Development Program[2019YFB1503904] ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources[SKLCUSA-b201802] ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion[Y907k51001]
WOS Research AreaMaterials Science
Funding OrganizationProgram of Science & Technology Planning Project of Xi' an city ; Natural Science Foundation of Shaanxi Province ; National Natural Science Foundation of China ; National Key Research and Development Program ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion
WOS SubjectMaterials Science, Paper & Wood
WOS IDWOS:000583802900074
PublisherNORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/34847
Collection中国科学院广州能源研究所
Corresponding AuthorXu, Huijuan
Affiliation1.Shaanxi Univ Sci & Technol, Coll Mech & Elect Engn, Xian 710021, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
First Author AffilicationGuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Yunyun,Xie, Xianli,Liu, Wenhuan,et al. Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237[J]. BIORESOURCES,2020,15(4):8355-8368.
APA Liu, Yunyun,Xie, Xianli,Liu, Wenhuan,Xu, Huijuan,&Cao, Yunqi.(2020).Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237.BIORESOURCES,15(4),8355-8368.
MLA Liu, Yunyun,et al."Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237".BIORESOURCES 15.4(2020):8355-8368.
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