Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper | |
| Zhang, Chunsun; Xu, Jinliang; Wang, Jianqin; Wang, Hanping | |
| 2007-02-01 | |
| Source Publication | ANALYTICAL LETTERS
 |
| Volume | 40Issue:3Pages:497-511 |
| Corresponding Author | xuil@ms.giec.ac.cn |
| Abstract | This paper presents a novel method for performing polymerase chain reaction (PCR) amplification by using spiral channel fabricated on copper where a transparent polytetrafluoroethylene ( PTFE) capillary tube was embedded. The channel with 25 PCR cycles was gradually developed in a spiral manner from inner to outer. The durations of PCR mixture at the denaturation, annealing and extension zones were gradually lengthened at a given flow rate, which may benefit continuous-flow PCR amplification as the synthesis ability of the Taq polymerase enzyme usually weakens with PCR time. Successful continuous-flow amplification of DNA fragments has been demonstrated. The PCR products of 249, 500 and 982 bp fragments could be obviously observed when the flow rates of PCR mixture were 7.5, 7.5 and 3.0 mm s(-1), respectively, and the required amplification times were about 25, 25, and 62 min, respectively. Besides, the successful segmented-flow PCR of three samples ( 249, 500 and 982 bp) has also been reported, which demonstrates the present continuous-flow PCR microfluidics can be developed for high-throughput genetic analysis. |
| Subtype | Article |
| Other Abstract | This paper presents a novel method for performing polymerase chain reaction (PCR) amplification by using spiral channel fabricated on copper where a transparent polytetrafluoroethylene ( PTFE) capillary tube was embedded. The channel with 25 PCR cycles was gradually developed in a spiral manner from inner to outer. The durations of PCR mixture at the denaturation, annealing and extension zones were gradually lengthened at a given flow rate, which may benefit continuous-flow PCR amplification as the synthesis ability of the Taq polymerase enzyme usually weakens with PCR time. Successful continuous-flow amplification of DNA fragments has been demonstrated. The PCR products of 249, 500 and 982 bp fragments could be obviously observed when the flow rates of PCR mixture were 7.5, 7.5 and 3.0 mm s(-1), respectively, and the required amplification times were about 25, 25, and 62 min, respectively. Besides, the successful segmented-flow PCR of three samples ( 249, 500 and 982 bp) has also been reported, which demonstrates the present continuous-flow PCR microfluidics can be developed for high-throughput genetic analysis. |
| Keyword | Continuous-flow Polymerase Chain Reaction Microfluidics Spiral Capillary Channel |
| WOS Headings | Science & Technology ; Physical Sciences |
| DOI | 10.1080/00032710600964775 |
| WOS Subject Extended | Chemistry |
| WOS Keyword | DNA AMPLIFICATION ; THROUGH PCR ; REACTION CHIP ; DEVICE ; SYSTEM ; ELECTROPHORESIS ; MICROCHIP ; TIME |
| Indexed By | SCI |
| Language | 英语 |
| WOS Subject | Chemistry, Analytical |
| WOS ID | WOS:000243120600008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/3464 |
| Collection | 中国科学院广州能源研究所 |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Micro Energy Syst Lab, Guangzhou 510640, Peoples R China 2.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Hefei 230026, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Chunsun,Xu, Jinliang,Wang, Jianqin,et al. Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper[J]. ANALYTICAL LETTERS,2007,40(3):497-511. |
| APA | Zhang, Chunsun,Xu, Jinliang,Wang, Jianqin,&Wang, Hanping.(2007).Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper.ANALYTICAL LETTERS,40(3),497-511. |
| MLA | Zhang, Chunsun,et al."Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper".ANALYTICAL LETTERS 40.3(2007):497-511. |
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