Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper | |
Zhang, Chunsun; Xu, Jinliang; Wang, Jianqin; Wang, Hanping | |
2007-02-01 | |
发表期刊 | ANALYTICAL LETTERS |
卷号 | 40期号:3页码:497-511 |
通讯作者 | xuil@ms.giec.ac.cn |
摘要 | This paper presents a novel method for performing polymerase chain reaction (PCR) amplification by using spiral channel fabricated on copper where a transparent polytetrafluoroethylene ( PTFE) capillary tube was embedded. The channel with 25 PCR cycles was gradually developed in a spiral manner from inner to outer. The durations of PCR mixture at the denaturation, annealing and extension zones were gradually lengthened at a given flow rate, which may benefit continuous-flow PCR amplification as the synthesis ability of the Taq polymerase enzyme usually weakens with PCR time. Successful continuous-flow amplification of DNA fragments has been demonstrated. The PCR products of 249, 500 and 982 bp fragments could be obviously observed when the flow rates of PCR mixture were 7.5, 7.5 and 3.0 mm s(-1), respectively, and the required amplification times were about 25, 25, and 62 min, respectively. Besides, the successful segmented-flow PCR of three samples ( 249, 500 and 982 bp) has also been reported, which demonstrates the present continuous-flow PCR microfluidics can be developed for high-throughput genetic analysis. |
文章类型 | Article |
其他摘要 | This paper presents a novel method for performing polymerase chain reaction (PCR) amplification by using spiral channel fabricated on copper where a transparent polytetrafluoroethylene ( PTFE) capillary tube was embedded. The channel with 25 PCR cycles was gradually developed in a spiral manner from inner to outer. The durations of PCR mixture at the denaturation, annealing and extension zones were gradually lengthened at a given flow rate, which may benefit continuous-flow PCR amplification as the synthesis ability of the Taq polymerase enzyme usually weakens with PCR time. Successful continuous-flow amplification of DNA fragments has been demonstrated. The PCR products of 249, 500 and 982 bp fragments could be obviously observed when the flow rates of PCR mixture were 7.5, 7.5 and 3.0 mm s(-1), respectively, and the required amplification times were about 25, 25, and 62 min, respectively. Besides, the successful segmented-flow PCR of three samples ( 249, 500 and 982 bp) has also been reported, which demonstrates the present continuous-flow PCR microfluidics can be developed for high-throughput genetic analysis. |
关键词 | Continuous-flow Polymerase Chain Reaction Microfluidics Spiral Capillary Channel |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1080/00032710600964775 |
研究领域[WOS] | Chemistry |
关键词[WOS] | DNA AMPLIFICATION ; THROUGH PCR ; REACTION CHIP ; DEVICE ; SYSTEM ; ELECTROPHORESIS ; MICROCHIP ; TIME |
收录类别 | SCI |
语种 | 英语 |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000243120600008 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.giec.ac.cn/handle/344007/3464 |
专题 | 中国科学院广州能源研究所 |
作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Micro Energy Syst Lab, Guangzhou 510640, Peoples R China 2.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Hefei 230026, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Chunsun,Xu, Jinliang,Wang, Jianqin,et al. Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper[J]. ANALYTICAL LETTERS,2007,40(3):497-511. |
APA | Zhang, Chunsun,Xu, Jinliang,Wang, Jianqin,&Wang, Hanping.(2007).Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper.ANALYTICAL LETTERS,40(3),497-511. |
MLA | Zhang, Chunsun,et al."Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper".ANALYTICAL LETTERS 40.3(2007):497-511. |
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