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Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper
Zhang, Chunsun; Xu, Jinliang; Wang, Jianqin; Wang, Hanping
2007-02-01
发表期刊ANALYTICAL LETTERS
卷号40期号:3页码:497-511
通讯作者xuil@ms.giec.ac.cn
摘要This paper presents a novel method for performing polymerase chain reaction (PCR) amplification by using spiral channel fabricated on copper where a transparent polytetrafluoroethylene ( PTFE) capillary tube was embedded. The channel with 25 PCR cycles was gradually developed in a spiral manner from inner to outer. The durations of PCR mixture at the denaturation, annealing and extension zones were gradually lengthened at a given flow rate, which may benefit continuous-flow PCR amplification as the synthesis ability of the Taq polymerase enzyme usually weakens with PCR time. Successful continuous-flow amplification of DNA fragments has been demonstrated. The PCR products of 249, 500 and 982 bp fragments could be obviously observed when the flow rates of PCR mixture were 7.5, 7.5 and 3.0 mm s(-1), respectively, and the required amplification times were about 25, 25, and 62 min, respectively. Besides, the successful segmented-flow PCR of three samples ( 249, 500 and 982 bp) has also been reported, which demonstrates the present continuous-flow PCR microfluidics can be developed for high-throughput genetic analysis.
文章类型Article
其他摘要This paper presents a novel method for performing polymerase chain reaction (PCR) amplification by using spiral channel fabricated on copper where a transparent polytetrafluoroethylene ( PTFE) capillary tube was embedded. The channel with 25 PCR cycles was gradually developed in a spiral manner from inner to outer. The durations of PCR mixture at the denaturation, annealing and extension zones were gradually lengthened at a given flow rate, which may benefit continuous-flow PCR amplification as the synthesis ability of the Taq polymerase enzyme usually weakens with PCR time. Successful continuous-flow amplification of DNA fragments has been demonstrated. The PCR products of 249, 500 and 982 bp fragments could be obviously observed when the flow rates of PCR mixture were 7.5, 7.5 and 3.0 mm s(-1), respectively, and the required amplification times were about 25, 25, and 62 min, respectively. Besides, the successful segmented-flow PCR of three samples ( 249, 500 and 982 bp) has also been reported, which demonstrates the present continuous-flow PCR microfluidics can be developed for high-throughput genetic analysis.
关键词Continuous-flow Polymerase Chain Reaction Microfluidics Spiral Capillary Channel
WOS标题词Science & Technology ; Physical Sciences
DOI10.1080/00032710600964775
研究领域[WOS]Chemistry
关键词[WOS]DNA AMPLIFICATION ; THROUGH PCR ; REACTION CHIP ; DEVICE ; SYSTEM ; ELECTROPHORESIS ; MICROCHIP ; TIME
收录类别SCI
语种英语
WOS类目Chemistry, Analytical
WOS记录号WOS:000243120600008
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/3464
专题中国科学院广州能源研究所
作者单位1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Micro Energy Syst Lab, Guangzhou 510640, Peoples R China
2.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Chunsun,Xu, Jinliang,Wang, Jianqin,et al. Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper[J]. ANALYTICAL LETTERS,2007,40(3):497-511.
APA Zhang, Chunsun,Xu, Jinliang,Wang, Jianqin,&Wang, Hanping.(2007).Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper.ANALYTICAL LETTERS,40(3),497-511.
MLA Zhang, Chunsun,et al."Continuous-flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper".ANALYTICAL LETTERS 40.3(2007):497-511.
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